In this work, a composite hydrogel material consisting of chitosan‐based composite hydrogel was prepared by a simple and rapid synthetic method and will be named three‐dimensional (3D)‐IL‐COF‐1@CS hydrogel. Possessing a stable 3D network structure and outstanding hydrophilicity, the novel hydrogel is capable of capturing glycopeptides. The 3D‐IL‐COF‐1@CS hydrogel showed good sensitivity (0.1 fmol/µL) and selectivity (1:2000). In addition, 19 glycopeptides were captured in standard samples. In the analysis of human serum, 148 glycopeptides assigned to 72 glycoproteins were assayed in the serum of normal individuals, and 245 glycopeptides corresponding to 100 glycoproteins were found in the serum of colorectal cancer (CRC) patients. More importantly, several functional programs based on Gene Ontology analysis supported molecular biological processes that may be relevant to the pathogenesis of CRC, including aging, fibrinogen complex, and arylesterase activity. The low cost, simplicity, rapid synthesis, and good enrichment performance have a great future in glycoproteomics analysis and related diseases.

中文翻译：

---

基于水凝胶聚合物的亲水材料用于富集结直肠癌患者血清中的糖肽


在这项工作中，通过简单快速的合成方法制备了一种由壳聚糖基复合水凝胶组成的复合水凝胶材料，并将其命名为三维（3D）-IL-COF-1@CS水凝胶。这种新型水凝胶具有稳定的3D网络结构和出色的亲水性，能够捕获糖肽。 3D-IL-COF-1@CS水凝胶表现出良好的灵敏度（0.1 fmol/μL）和选择性（1:2000）。此外，在标准样品中还捕获了 19 种糖肽。在对人血清的分析中，在正常个体的血清中检测到了对应于72种糖蛋白的148种糖肽，并且在结直肠癌（CRC）患者的血清中发现了对应于100种糖蛋白的245种糖肽。更重要的是，基于基因本体分析的几个功能程序支持可能与CRC发病机制相关的分子生物学过程，包括衰老、纤维蛋白原复合物和芳基酯酶活性。其成本低廉、操作简单、合成快速、富集性能好，在糖蛋白组学分析及相关疾病方面具有广阔的前景。