Background Given the significant impact on human health, it is imperative to develop novel treatment approaches for diabetic wounds, which are prevalent and serious complications of diabetes. The diabetic wound microenvironment has a high level of reactive oxygen species (ROS) and an imbalance between proinflammatory and anti-inflammatory cells/factors, which hamper the healing of chronic wounds. This study aimed to develop poly(L-lactic acid) (PLLA) nanofibrous membranes incorporating curcumin and silver nanoparticles (AgNPs), defined as PLLA/C/Ag, for diabetic wound healing. Methods PLLA/C/Ag were fabricated via an air-jet spinning approach. The membranes underwent preparation and characterization through various techniques including Fourier-transform infrared spectroscopy, measurement of water contact angle, X-ray photoelectron spectroscopy, X-ray diffraction, scanning electron microscopy, assessment of in vitro release of curcumin and Ag+, testing of mechanical strength, flexibility, water absorption and biodegradability. In addition, the antioxidant, antibacterial and anti-inflammatory properties of the membranes were evaluated in vitro, and the ability of the membranes to heal wounds was tested in vivo using diabetic mice. Results Loose hydrophilic nanofibrous membranes with uniform fibre sizes were prepared through air-jet spinning. The membranes enabled the efficient and sustained release of curcumin. More importantly, antibacterial AgNPs were successfully reduced in situ from AgNO3. The incorporation of AgNPs endowed the membrane with superior antibacterial activity, and the bioactivities of curcumin and the AgNPs gave the membrane efficient ROS scavenging and immunomodulatory effects, which protected cells from oxidative damage and reduced inflammation. Further results from animal studies indicated that the PLLA/C/Ag membranes had the most efficient wound healing properties, which were achieved by stimulating angiogenesis and collagen deposition and inhibiting inflammation. Conclusions In this research, we successfully fabricated PLLA/C/Ag membranes that possess properties of antioxidants, antibacterial agents and anti-inflammatory agents, which can aid in the process of wound healing. Modulating wound inflammation, these new PLLA/C/Ag membranes serve as a novel dressing to enhance the healing of diabetic wounds.

中文翻译：

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免疫调节聚（L-乳酸）纳米纤维膜通过抑制炎症、氧化和细菌感染来促进糖尿病伤口愈合。


背景 鉴于糖尿病伤口对人类健康的重大影响，迫切需要开发新的治疗方法来治疗糖尿病伤口，糖尿病伤口是糖尿病普遍且严重的并发症。糖尿病伤口微环境具有高水平的活性氧（ROS）以及促炎和抗炎细胞/因子之间的不平衡，这阻碍了慢性伤口的愈合。本研究旨在开发包含姜黄素和银纳米粒子 (AgNP) 的聚（L-乳酸）（PLLA）纳米纤维膜（定义为 PLLA/C/Ag），用于糖尿病伤口愈合。方法通过喷气纺丝方法制备PLLA/C/Ag。通过傅里叶变换红外光谱、水接触角测量、X射线光电子能谱、X射线衍射、扫描电子显微镜、姜黄素和Ag+的体外释放评估、机械性能测试等多种技术对膜进行了制备和表征。强度、柔韧性、吸水性和生物降解性。此外，还对膜的抗氧化、抗菌和抗炎特性进行了体外评估，并使用糖尿病小鼠体内测试了膜愈合伤口的能力。结果通过喷气纺丝制备出纤维尺寸均匀的疏松亲水纳米纤维膜。该膜能够有效且持续地释放姜黄素。更重要的是，抗菌AgNPs 成功地从AgNO3 中原位还原。 AgNPs的掺入赋予膜优异的抗菌活性，姜黄素和AgNPs的生物活性赋予膜有效的ROS清除和免疫调节作用，从而保护细胞免受氧化损伤并减少炎症。 动物研究的进一步结果表明，PLLA/C/Ag 膜具有最有效的伤口愈合特性，这是通过刺激血管生成和胶原蛋白沉积以及抑制炎症来实现的。结论 在本研究中，我们成功制备了具有抗氧化剂、抗菌剂和抗炎剂特性的 PLLA/C/Ag 膜，有助于伤口愈合过程。这些新型 PLLA/C/Ag 膜可调节伤口炎症，作为一种新型敷料，可促进糖尿病伤口的愈合。