Mentha haplocalyx essential oil (MEO) has demonstrated inhibitory effects on Fusarium oxysporum. Despite its environmentally friendly properties as a natural product, the limited water solubility of MEO restricts its practical application in the field. The use of nanoemulsion can improve bioavailability and provide an eco-friendly approach to prevent and control Panax notoginseng root rot. In this study, Tween 80 and anhydrous ethanol (at a mass ratio of 3) were selected as carriers, and the ultrasonic method was utilized to produce a nanoemulsion of MEO (MNEO) with an average particle size of 26.07 nm. Compared to MTEO (MEO dissolved in an aqueous solution of 2% DMSO and 0.1% Tween 80), MNEO exhibited superior inhibition against F. oxysporum in terms of spore germination and hyphal growth. Transcriptomics and metabolomics results revealed that after MNEO treatment, the expression levels of certain genes related to glycolysis/gluconeogenesis, starch and sucrose metabolism were significantly suppressed along with the accumulation of metabolites, leading to energy metabolism disorder and growth stagnation in F. oxysporum. In contrast, the inhibitory effect from MTEO treatment was less pronounced. Furthermore, MNEO also demonstrated inhibition on meiosis, ribosome function, and ribosome biogenesis in F. oxysporum growth process. These findings suggest that MNEO possesses enhanced stability and antifungal activity, which effectively hinders F. oxysporum through inducing energy metabolism disorder, meiotic stagnation, as well as ribosome dysfunction, thus indicating its potential for development as a green pesticide for prevention and control P. notoginseng root rot caused by F.oxyosporum.

薄荷精油 (MEO) 对尖孢镰刀菌具有抑制作用。尽管MEO作为天然产物具有环保特性，但其有限的水溶性限制了其在该领域的实际应用。纳米乳的使用可以提高生物利用度，为防治三七根腐病提供一种生态友好的方法。本研究选择吐温80和无水乙醇（质量比为3）为载体，采用超声法制备平均粒径为26.07 nm的MEO（MNEO）纳米乳液。与MTEO（溶解在2％DMS​​O和0.1％Tween 80的水溶液中的MEO）相比，MNEO在孢子萌发和菌丝生长方面对尖孢镰刀菌表现出优异的抑制作用。转录组学和代谢组学结果表明，经MNEO处理后，尖孢镰刀菌某些与糖酵解/糖异生、淀粉和蔗糖代谢相关的基因表达水平显着受到抑制，代谢物不断积累，导致尖孢镰刀菌能量代谢紊乱和生长停滞。相比之下，MTEO 处理的抑制作用不太明显。此外，MNEO 还表现出对尖孢镰刀菌生长过程中减数分裂、核糖体功能和核糖体生物合成的抑制作用。这些结果表明，MNEO具有增强的稳定性和抗真菌活性，可通过诱导能量代谢紊乱、减数分裂停滞以及核糖体功能障碍来有效阻碍尖孢镰刀菌，从而表明其作为防治尖孢镰刀菌的绿色农药的潜力。 由尖孢镰刀菌引起的三七根腐病。