In the wake of global population increases and stagnating capture fishery production, there exists a requirement for novel biotechnologies to increase aquaculture productivity to offset demand. Through recognition of limitations imposed by aquaculture—increased incidence of disease, stress, cannibalism, and mortality in captivity—researchers continue to investigate RNA interference (RNAi) and clustered regularly interspaced short palindromic repeat (CRISPR) technologies for their potential to alleviate these at a genetic level. In decapods, with the exception of generating desirable all-male monosex populations in Macrobrachium rosenbergii, RNAi and CRISPR technologies have yet to provide a significant commercial use case. This literature review provides a comprehensive examination of RNAi and CRISPR in decapods, along with popular delivery methods (injection/microinjection; electroporation; receptor-mediated ovary transduction of cargo [ReMOT]; and nanoparticles). It identifies a number of limitations including scalability bottlenecks imposed by RNAi delivery methods, and deferred gene editing progress as a result of CRISPR's infancy. Finally, it recognises gene editing induced gene silencing (GEiGS®) as a novel method for silencing gene expression, and suggests an interim examination of transgenerational gene silencing (TGS) (‘parental RNAi’) in decapods to enhance aquaculture productivity.

中文翻译：

---

评估跨代基因编辑能力以提高十足目甲壳类动物的水产养殖生产力


随着全球人口增加和捕捞渔业生产停滞，需要新型生物技术来提高水产养殖生产力以抵消需求。通过认识到水产养殖带来的局限性（圈养条件下疾病、应激、同类相食和死亡率增加），研究人员继续研究 RNA 干扰 (RNAi) 和成簇规则间隔短回文重复 (CRISPR) 技术，以了解它们在一定程度上缓解这些问题的潜力。遗传水平。在十足目动物中，除了在罗氏沼虾中产生理想的全雄性单性种群外，RNAi 和 CRISPR 技术尚未提供重要的商业用例。本文献综述对十足目动物中的 RNAi 和 CRISPR 以及流行的递送方法（注射/显微注射、电穿孔、受体介导的卵巢货物转导 [ReMOT] 和纳米颗粒）进行了全面检查。它指出了许多限制，包括 RNAi 传递方法带来的可扩展性瓶颈，以及由于 CRISPR 尚处于起步阶段而推迟的基因编辑进展。最后，它认识到基因编辑诱导基因沉默（GEiGS®）是一种沉默基因表达的新方法，并建议对十足目动物中的跨代基因沉默（TGS）（“亲代RNAi”）进行中期检查，以提高水产养殖生产力。