BackgroundIt is reported that CD123 + HLA-DR- cells in PBMC are basophils, and CD203c, CD63, and FcεRI molecules are activation markers of basophils. However, little is known of CD123 + HLA-DR-cells in blood granulocytes.ObjectiveTo investigate the presence of CD123 + HLA-DR- cells in the blood granulocytes and peripheral PBMC of patients with allergic rhinitis (AR), as well as the impact of allergens on the cell membrane markers of basophils.MethodsFlow cytometry was used to detect the expression of the membrane molecules.ResultsWhile CD123 + HLA-DR- PBMCs are representative of basophils, their presence did not significantly change in patients with AR. In contrast, both the percentage and number of CD123 + HLA-DR- granulocytes, which make up only up to 50% of basophils, were significantly increased in patients with seasonal (sAR) and perennial AR (pAR). CD63+, CD203c+, and FcεRIα+ cells within CD123 + HLA-DR- granulocytes also showed enhanced activity in patients with AR. Allergen extracts from house dust mite allergen extract (HDME) and Artemisia sieversiana wild extract further increased the number of CD123 + HLA-DR- cells in granulocytes of sAR and pAR patients, as well as in PBMCs of pAR patients.ConclusionsThe use of CD123 + HLA-DR- granulocytes and PBMC may not be sufficient for diagnosing AR. Allergens could potentially contribute to the development of AR by influencing the number of CD123 + HLA-DR- cells, as well as the expression of CD63, CD203c, and FcεRIαin these cells.

中文翻译：

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变应性鼻炎中CD123+HLA-DR−粒细胞的增加及变应原对细胞膜标志物表达的影响


背景据报道，PBMC中的CD123+HLA-DR-细胞为嗜碱性粒细胞，CD203c、CD63、FcεRI分子为嗜碱性粒细胞的激活标志物。然而，人们对血液粒细胞中CD123+HLA-DR-细胞的了解甚少。目的探讨变应性鼻炎(AR)患者血液粒细胞和外周PBMC中CD123+HLA-DR-细胞的存在情况及其影响。方法采用流式细胞术检测膜分子的表达。结果虽然CD123+HLA-DR-PBMCs是嗜碱性粒细胞的代表，但其存在在AR患者中没有显着变化。相比之下，季节性 (sAR) 和常年性 AR (pAR) 患者的 CD123 + HLA-DR- 粒细胞（仅占嗜碱性粒细胞的 50%）的百分比和数量均显着增加。 AR 患者中 CD123 + HLA-DR- 粒细胞内的 CD63+、CD203c+ 和 FcεRIα+ 细胞也表现出增强的活性。屋尘螨过敏原提取物 (HDME) 和大白蒿野生提取物中的过敏原提取物进一步增加了 sAR 和 pAR 患者的粒细胞以及 pAR 患者的 PBMC 中 CD123 + HLA-DR- 细胞的数量。结论 CD123 + 的使用HLA-DR-粒细胞和 PBMC 可能不足以诊断 AR。过敏原可能通过影响 CD123 + HLA-DR- 细胞的数量以及这些细胞中 CD63、CD203c 和 FcεRIα 的表达来潜在地促进 AR 的发展。