评估咖啡双耳对食源性病原体和淡色库蚊的化学特征和潜在生物防治，以确保食品安全,Journal of Food Safety

当前位置： X-MOL 学术 › J. Food Saf. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

评估咖啡双耳对食源性病原体和淡色库蚊的化学特征和潜在生物防治，以确保食品安全
Journal of Food Safety ( IF 1.9 ) Pub Date : 2024-04-16 , DOI: 10.1111/jfs.13124
Doaa R. Abdel‐Haleem ₁ , Diaa A. Marrez ₂ , Mohamed A. El Raey ₃ , Mahmoud Emam ₃ , Mohamed Seif ₂ , Shaimaa M. Farag ₁

Affiliation

广泛使用合成抗生素和杀虫剂来控制微生物和昆虫传播，导致耐药菌株的积累，并通过食品和环境中的生物累积对人类健康造成负面影响。本研究评估了抗菌活性咖啡双耳瓶（咖啡形阿.）乙醇提取物对抗食源性致病微生物并评估其控制能力淡色库蚊L.（尖色隐球菌）幼虫。气相色谱-质谱（GC/MS）和高效液相色谱（HPLC）分析表明咖啡形阿.提取物富含酚类化合物、有机硫化合物、羧酸、氨基酸、有机喹啉、二肽和单糖。的提取物咖啡形阿.对所有测试的细菌均表现出抗菌能力，最低抑制浓度 (MIC) 值范围为 20.2 至 66.7 g/mL，并且对所有测试的产霉菌毒素真菌均具有抗真菌作用，MIC 值范围为 113.62、68.95 和 49.37 μg/mL mg /毫升。此外，该提取物对幼虫表现出很高的杀幼活性。尖色隐球菌分别在 24、48 和 72 h 时观察幼虫。液相色谱50值随治疗时间逐渐下降。同样，成虫的羽化和生长指数与提取物浓度的增加呈显着负相关。此外，该提取物表现出显着的生殖潜力消耗和不育指数增加。尖色隐球菌经处理的幼虫发育成雌性。除了降低卵孵化率外，还会增加蛹畸形、增加成虫畸形。尖色隐球菌生化标记；谷胱甘肽-S提取物暴露后，转移酶、羧酸酯酶、乙酰胆碱酯酶、α-酯酶和细胞色素 P-450 显示出显着变化。总的来说，这些发现预示着应用的可能性咖啡形阿.提取物可作为对抗食源性病原体的生物防腐剂，也可作为控制蚊子的生物杀虫剂。

"点击查看英文标题和摘要"

Assessment of the chemical profile and potential biocontrol of Amphora coffeaeformis against foodborne pathogens and Culex pipiens L. to ensure food safety

The extensive use of synthetic antibiotics and insecticides in controlling microbes and insect spreads led to a build‐up of resistance strains and caused negative impacts on human health through bioaccumulation in food and the environment. The present study assessed the antimicrobial activity of Amphora coffeaeformis (A. coffeaeformis) ethanolic extract against foodborne pathogenic microbes as well as assessed its ability to control Culex pipiens L. (C. pipiens) larvae. The gas chromatography–mass spectrum (GC/MS) and high‐performance liquid chromatography (HPLC) analysis showed that A. coffeaeformis extract was rich with phenolic compounds, organosulfur compounds, carboxylic acids, amino acids, organic quinoline, dipeptide and monosaccharide. The extract of A. coffeaeformis showed antibacterial capability against all tested bacteria, with minimal inhibition concentration (MIC) values ranging from 20.2 to 66.7 g/mL, as well as antifungal effects against all tested mycotoxigenic fungi, with MIC values ranging from 113.62, 68.95 and 49.37 μg/mL mg/mL. Furthermore, the extract showed high larvicidal activity against C. pipiens larvae at 24, 48, and 72 h, respectively. LC50 values decreased gradually with the treatment period. Likewise, the adult emergence and growth index were significantly negatively related to increasing extract concentrations. Moreover, the extract exhibited a noteworthy depletion in the reproductive potential and increased sterility index of C. pipiens females developed of treated larvae. Besides a reduction in egg hatching percent, increase pupal malformations, increase and adult abnormalities. C. pipiens biochemical markers; glutathione‐S‐transferase, carboxylesterase, acetylcholinesterase, α‐esterase and cytochrome P‐450 showed significant alteration after the extract exposure. Overall, these findings promise the application possibility of A. coffeaeformis extract as a biopreservator against foodborne pathogens and as bioinsecticides for mosquito control as well.

更新日期：2024-04-16

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南