<br>Geraniin 通过靶向 IL-1β 减轻 Caco-2 细胞和葡聚糖硫酸钠诱导的结肠炎小鼠的炎症,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Geraniin 通过靶向 IL-1β 减轻 Caco-2 细胞和葡聚糖硫酸钠诱导的结肠炎小鼠的炎症
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-03-26 , DOI: 10.1021/acs.jafc.3c09396
Hae-Ri Lee ₁ , Young-Jin Jeong ₁ , Sun-Ae Park ₁ , Hee Jung Kim ₁ , Tae-Hwe Heo ₁

Affiliation

IL-1β 是一种重要的细胞因子，与炎症性肠病 (IBD) 和肠道屏障功能障碍的进展有关。多酚化合物香叶苷具有抗肿瘤、抗氧化、抗炎、降压、抗病毒等生物活性；然而，其针对 IL-1β 的抗结肠炎活性仍不清楚。在这里，我们评估了香叶素对 IL-1β 刺激的 Caco-2 细胞和葡聚糖硫酸钠 (DSS) 诱导的结肠炎小鼠模型的抑制作用。 Geraniin 通过直接与 IL-1β 结合，阻断 IL-1β 和 IL-1R 之间的相互作用，抑制 IL-1β 活性。它通过抑制 IL-1β 介导的 MAPK、NF-kB 和 MLC 激活来抑制 IL-1β 诱导的人 Caco-2 细胞肠道紧密连接损伤。此外，天竺葵苷通过抑制 MAPK、NF-kB 和 MLC 激活来抑制肠道通透性升高并恢复紧密连接蛋白表达，从而有效减轻小鼠结肠炎症状并减轻肠道屏障损伤。因此，香叶素通过阻碍 IL-1β 和 IL-1R 相互作用而表现出抗 IL-1β 活性和抗结肠炎作用，并且可能是用于 IBD 治疗的有前途的治疗性抗 IL-1β 药物。

"点击查看英文标题和摘要"

Geraniin Alleviates Inflammation in Caco-2 Cells and Dextran Sulfate Sodium-Induced Colitis Mice by Targeting IL-1β

IL-1β is an important cytokine implicated in the progression of inflammatory bowel disease (IBD) and intestinal barrier dysfunction. The polyphenolic compound, geraniin, possesses bioactive properties, such as antitumor, antioxidant, anti-inflammatory, antihypertensive, and antiviral activities; however, its IL-1β-targeted anticolitis activity remains unclear. Here, we evaluated the inhibitory effect of geraniin in IL-1β-stimulated Caco-2 cells and a dextran sulfate sodium (DSS)-induced colitis mouse model. Geraniin blocked the interaction between IL-1β and IL-1R by directly binding to IL-1β and inhibited the IL-1β activity. It suppressed IL-1β-induced intestinal tight junction damage in human Caco-2 cells by inhibiting IL-1β-mediated MAPK, NF-kB, and MLC activation. Moreover, geraniin administration effectively reduced colitis symptoms and attenuated intestinal barrier injury in mice by suppressing elevated intestinal permeability and restoring tight junction protein expression through the inhibition of MAPK, NF-kB, and MLC activation. Thus, geraniin exhibits anti-IL-1β activity and anticolitis effect by hindering the IL-1β and IL-1R interaction and may be a promising therapeutic anti-IL-1β agent for IBD treatment.

更新日期：2024-03-26

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南