Collagen-I (Col) and hyaluronic acid (HA) are two of the most important extracellular matrix (ECM) components. While collagen serves as the main structural feature of the ECM and is an important ligand for integrin-based adhesions, HA acts as a ligand for the transmembrane glycoprotein CD44. However, most studies on cancer invasion utilize 3D collagen matrices, thereby ignoring the importance of HA-CD44 interactions. Here, we describe the process of fabricating Col-HA gels with varying Col/HA ratios, which can be used to suitably mimic the tumor microenvironment.

中文翻译：

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胶原-透明质酸 (Col-HA) 基质的制备和表征：肿瘤微环境的体外模拟。


I 型胶原蛋白 (Col) 和透明质酸 (HA) 是两种最重要的细胞外基质 (ECM) 成分。胶原蛋白是 ECM 的主要结构特征，并且是基于整合素的粘连的重要配体，而 HA 则充当跨膜糖蛋白 CD44 的配体。然而，大多数关于癌症侵袭的研究都利用 3D 胶原蛋白基质，从而忽略了 HA-CD44 相互作用的重要性。在这里，我们描述了制造具有不同 Col/HA 比例的 Col-HA 凝胶的过程，该凝胶可用于适当地模拟肿瘤微环境。