Precise determination of the carbamate pesticide carbosulfan is crucial for assessing the associated risks in food and environment. Due to the strong interaction between carbosulfan and target enzyme, current methods primarily depend on the acetylcholinesterase (AChE) inhibition strategy, which generally lacks selectivity. In this study, we propose a nanozyme colorimetric sensor for the specific carbosulfan detection, based on its distinctive hydrolysis property. In contrast to other pesticides, carbosulfan can be hydrolyzed to produce the reductive sulfide compound by the cleavage of N–S bond under acidic condition, thereby significantly hindering the nanozyme-mediated chromogenic reaction. Consequently, the absorbance is significantly correlated with carbosulfan concentration. Furthermore, the influence of nanozyme type is disclosed, and two oxidase-like carbon nanozymes were formulated, namely metal-free NC and metal-based CeO₂@NC. However, the distinct active sites significantly impact the proposed sensor. For CeO₂@NC-based sensor, the produced sulfide compounds not only poison Ce active site, but also consume the reactive oxygen species, thereby, exhibiting high sensitivity with low detection limit of 3.3 nM. By contrast, the metal-free nature of NC allows the assay to remain unaffected by coordination effects, exhibiting superior anti-interference capability. This work not only offers an efficient alternative to the conventional method for detecting carbosulfan specifically, but also shed light on the role of metal-based or metal-free nanozyme among analytical applications.

精确测定氨基甲酸酯类农药丁硫克百威对于评估食品和环境中的相关风险至关重要。由于克百威与靶酶之间的强烈相互作用，目前的方法主要依赖于乙酰胆碱酯酶（AChE）抑制策略，该策略通常缺乏选择性。在本研究中，我们基于其独特的水解特性，提出了一种用于特定丁硫克百威检测的纳米酶比色传感器。与其他农药相比，克百威在酸性条件下可通过N-S键断裂而水解生成还原性硫化物，从而显着阻碍纳米酶介导的显色反应。因此，吸光度与丁硫克百威浓度显着相关。此外，还揭示了纳米酶类型的影响，并配制了两种类氧化酶碳纳米酶，即无金属NC和金属基CeO ₂ @NC。然而，不同的活性位点显着影响所提出的传感器。对于基于CeO ₂ @NC的传感器，产生的硫化物不仅毒害Ce活性位点，而且消耗活性氧，从而表现出高灵敏度和3.3 nM的低检测限。相比之下，NC 的不含金属性质使测定不受配位效应的影响，表现出卓越的抗干扰能力。这项工作不仅为专门检测克百威的传统方法提供了一种有效的替代方法，而且还阐明了基于金属或不含金属的纳米酶在分析应用中的作用。