Regulation on Pathway Metabolic Fluxes to Enhance Colanic Acid Production in Escherichia coli,Journal of Agricultural and Food Chemistry

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Regulation on Pathway Metabolic Fluxes to Enhance Colanic Acid Production in Escherichia coli
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-09-09 , DOI: 10.1021/acs.jafc.3c05046
Wenxian Liu ₁ , Jing Peng ₁ , Sini Zou ₁ , Liting Xu ₁ , Haina Cheng _{1,

2} , Yuguang Wang _{1,

2} , Zhu Chen _{1,

2} , Hongbo Zhou _{1,

2}

Affiliation

Colanic acid (CA) is a natural polysaccharide macromolecule with rich and unique biological properties and is a promising candidate for use in food and cosmetics. To date, the efficient biosynthesis of CA and the influence of product accumulation on the strains used have yet to be precisely investigated. Herein, bottlenecks in the CA metabolic pathway were untangled by finely regulating the expression of manA, cpsG, fcl, and rcsA. Engineered strains produced CA at >1 g/L in shake flasks without dependence on cold temperatures, and it was verified in a 1 L bioreactor with a titer up to 18.64 g/L within 24 h. The accumulation of CA caused a decrease in the saturated fatty acid content (represented by C_16:0 and C_18:0) in the cell membrane. This study demonstrated pathway engineering for efficient CA production in cell factories and provided insights into the barriers and solutions faced in the biosynthesis of natural products.

中文翻译：

调节途径代谢通量以增强大肠杆菌中的可拉酸产量

豆蔻酸（CA）是一种天然多糖大分子，具有丰富而独特的生物学特性，是食品和化妆品中很有前景的候选者。迄今为止，CA 的有效生物合成以及产物积累对所用菌株的影响尚未得到精确研究。在此，通过精细调节manA、cpsG、fcl和rcsA的表达，解开了 CA 代谢途径中的瓶颈。工程菌株在摇瓶中不依赖于低温而产生 >1 g/L 的 CA，并在 1 L 生物反应器中得到验证，24 小时内滴度高达 18.64 g/L。CA的积累导致细胞膜中饱和脂肪酸含量（以C _16:0和C _{18:0表示）减少。}这项研究展示了细胞工厂中高效 CA 生产的途径工程，并提供了对天然产物生物合成中面临的障碍和解决方案的见解。

更新日期：2023-09-09

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