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“抗生素耐药性、生物膜形成、毒力因子和铜绿假单胞菌环境分离株来源与金属-β-内酰胺酶编码基因存在之间的关系”
Microbial Pathogenesis ( IF 3.3 ) Pub Date : 2023-07-07 , DOI: 10.1016/j.micpath.2023.106223 Letícia Franco Gervasoni 1 , Inaiá Calegari Peixoto 1 , Anna Cardoso Imperador 1 , Lívia Batista De Oliveira 1 , Lucimeire Fernandes Correia 2 , Karolinny Cristiny de Oliveira Vieira 3 , Erika Kushikawa Saeki 4 , Paula Esteves da Silva Lima 4 , Edson Assunção Mareco 5 , Valéria Cataneli Pereira 6 , Lizziane Kretli Winkelströter Eller 7
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更新日期:2023-07-10
Microbial Pathogenesis ( IF 3.3 ) Pub Date : 2023-07-07 , DOI: 10.1016/j.micpath.2023.106223 Letícia Franco Gervasoni 1 , Inaiá Calegari Peixoto 1 , Anna Cardoso Imperador 1 , Lívia Batista De Oliveira 1 , Lucimeire Fernandes Correia 2 , Karolinny Cristiny de Oliveira Vieira 3 , Erika Kushikawa Saeki 4 , Paula Esteves da Silva Lima 4 , Edson Assunção Mareco 5 , Valéria Cataneli Pereira 6 , Lizziane Kretli Winkelströter Eller 7
Affiliation
总共评估了 557 个水样,其中 23 个水样呈铜绿假单胞菌阳性。其中大约 91.7% 是弱生物膜形成者。只有 4 个分离株表现出抗菌药物耐药性。所有分离株均表现出抽搐运动,这是绿脓素、碱性蛋白酶和溶血素产生的阳性结果。基因型测试显示: lasA (95.6%) lasB (95.6%)、exoS (95.6%)、exoT (91.3%)、toxA (91.3%)、akgO (91.3%)、plcN (91.3%) aprA (86.9%) )、phzM (78.3%) 和pvdA (60.9%)。对于编码金属-β-内酰胺酶的基因,发现:bla VIM (56.6%)、bla SPM (4.3%) 和bla SIM (47.8%)。发现金属-β-内酰胺酶产生基因、九个毒力因子基因和运动性之间存在很强的关联(r = 0.6231)。非常接近的克隆特征表明来自不同城市的分离株之间可能存在相似性。因此,铜绿假单胞菌可能存在于具有不同毒力能力的供水中,并且可能引起对人类、动物和环境健康的巨大关注。
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