细胞表面糖蛋白组学：通过独特的分析捕获方法破译糖蛋白,Analytical Methods

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细胞表面糖蛋白组学：通过独特的分析捕获方法破译糖蛋白
Analytical Methods ( IF 2.7 ) Pub Date : 2023-06-26 , DOI: 10.1039/d3ay00982c
Shan Huang ₁ , Xiaotong Wang ₂ , Peng Zhang ₃ , Shuang Yang _{1,

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Affiliation

细胞表面蛋白（CSP）通常参与各种生物过程，例如细胞间相互作用、免疫反应和分子运输。CSP的异常表达通常预示着人类疾病的发生和发展。大多数 CSP 都是糖基化的，并已被探索作为潜在的药物靶点和疾病生物标志物；然而，由于 CSP 丰度低且疏水性强，从细胞内蛋白质中有效分离 CSP 很困难。表面糖蛋白的全面表征仍然是一个巨大的挑战，并且在蛋白质组学中往往代表性不足。近年来，表面蛋白的质谱分析取得了前所未有的进展，CSP捕获方法和质谱技术得到了很大的发展。在本文中，我们的目标是全面概述可丰富 CSP 的创新分析方法，包括基于离心的分离、相分配、基于粘附的表面蛋白捕获、抗体或凝集素亲和力以及基于生物素的化学标记。通过聚糖的化学氧化或碳水化合物代谢标记的点击化学来捕获表面糖蛋白。这些技术为研究细胞表面受体的功能和识别诊断和治疗开发的标记物提供了广泛的应用。通过聚糖的化学氧化或碳水化合物代谢标记的点击化学来捕获表面糖蛋白。这些技术为研究细胞表面受体的功能和识别诊断和治疗开发的标记物提供了广泛的应用。通过聚糖的化学氧化或碳水化合物代谢标记的点击化学来捕获表面糖蛋白。这些技术为研究细胞表面受体的功能和识别诊断和治疗开发的标记物提供了广泛的应用。

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Cell surface glycoproteomics: deciphering glycoproteins through a unique analytical capture approach

Cell surface proteins (CSPs) are often involved in various biological processes such as cell–cell interactions, immune responses, and molecular transport. The abnormal expression of CSP usually indicates the occurrence and development of human diseases. Most CSPs are glycosylated and have been explored as potential drug targets and disease biomarkers; however, efficient isolation of CSPs from intracellular proteins is difficult due to their low abundance and strong hydrophobicity. The comprehensive characterization of surface glycoproteins remains a great challenge and is often underrepresented in proteomics. In recent years, unprecedented progress has been made in the mass spectrometry analysis of surface proteins, and CSP capture methods and mass spectrometry have been greatly developed. In this article, we aim to give a comprehensive overview of innovative analytical methods that can enrich CSPs, including centrifugation-based separation, phase partitioning, adhesion-based capture of surface proteins, antibody or lectin affinity, and biotin-based chemical labeling. Surface glycoproteins are captured by chemical oxidation of glycans or click chemistry for carbohydrate metabolic labeling. These techniques offer a wide range of applications for studying the function of cell surface receptors and identifying markers for diagnostic and therapeutic development.

更新日期：2023-06-30

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