根据锌和铁的总含量及其 Caco-2 细胞的吸收筛选八个生物强化水稻品种：体外细胞模型,ACS Food Science & Technology

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根据锌和铁的总含量及其 Caco-2 细胞的吸收筛选八个生物强化水稻品种：体外细胞模型
ACS Food Science & Technology ( IF 2.6 ) Pub Date : 2023-05-17 , DOI: 10.1021/acsfoodscitech.3c00031
Sandip Nathani ₁ , C. N. Neeraja ₂ , R. M. Sundaram ₂ , Partha Roy ₁

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锌（Zn）和铁（Fe）是两种必需的微量营养素，有助于人体维持正常的生理和新陈代谢。水稻是最重要的主粮作物，因为它养育着世界上大约一半的人口。本研究旨在筛选八个水稻品种的总谷物锌和铁含量以及它们在分化的 Caco-2 细胞中的吸收。使用电感耦合等离子体质谱 (ICP-MS) 技术测定谷物中 Zn 和 Fe 的总含量及其生物可及性。基于 Caco-2 细胞的荧光测定和铁蛋白合成测定分别用于测定 Zn 和 Fe 的吸收。已在 Caco-2 细胞中分析了微量营养素转运蛋白的转录表达，以响应体外消化的稻米样品中 Zn 和 Fe 的吸收。这项研究的结果揭示了对八个不同品种精米中两种主要微量营养素含量的比较分析。DRRDhan 48 (4.93 ± 0.10 mg/kg)、Zinco Rice (4.65 ± 0.01 mg/kg) 和 Protozin (4.43 ± 0.03 mg/kg) 具有低植酸含量和高谷物锌含量 (32.49 ± 2.8，分别为 27.9 ± 1.8、26.7 ± 1.2 mg/kg），并且分化的 Caco-2 细胞中锌的吸收显着增加。这项研究的结果为根据微量营养素的吸收筛选食品或谷物提供了一种简单方便的方法。和高谷物锌含量（分别为 32.49 ± 2.8、27.9 ± 1.8、26.7 ± 1.2 mg/kg），以及分化的 Caco-2 细胞中锌的吸收显着增加。这项研究的结果为根据微量营养素的吸收筛选食品或谷物提供了一种简单方便的方法。和高谷物锌含量（分别为 32.49 ± 2.8、27.9 ± 1.8、26.7 ± 1.2 mg/kg），以及分化的 Caco-2 细胞中锌的吸收显着增加。这项研究的结果为根据微量营养素的吸收筛选食品或谷物提供了一种简单方便的方法。

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Screening of Eight Biofortified Rice Varieties Based on Total Content of Zinc and Iron as Well as Its Uptake by Caco-2 Cells: An In Vitro Cellular Model

Zinc (Zn) and iron (Fe) are two essential micronutrients that help the body to maintain its normal physiology and metabolism. Rice is the most important staple crop because it nourishes approximately half of the world’s population. The present study was intended to screen a set of eight rice varieties for total grain Zn and Fe content along with their uptake in differentiated Caco-2 cells. The total grain Zn and Fe content, as well as their bioaccessibility, was determined using inductively coupled plasma mass spectrometry (ICP-MS) techniques. The Caco-2 cell-based fluorescence assays and ferritin synthesis assays were used to determine Zn and Fe uptake, respectively. The transcriptional expression of micronutrient transporters has been analyzed in Caco-2 cells in response to the uptake of Zn and Fe from in vitro digested rice samples. The findings of this study revealed a comparative analysis of two prominent micronutrient contents in eight different varieties of polished rice. DRRDhan 48 (4.93 ± 0.10 mg/kg), Zinco Rice (4.65 ± 0.01 mg/kg), and Protozin (4.43 ± 0.03 mg/kg) were shown to have low phytate content, and high grain Zn content (32.49 ± 2.8, 27.9 ± 1.8, 26.7 ± 1.2 mg/kg, respectively), as well as significantly greater Zn uptake in differentiated Caco-2 cells. The outcome of this study provides an easy and convenient approach for the screening of food items or cereals based on micronutrient uptake.

更新日期：2023-05-17

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