通过 LPS 和 IL-4 同时刺激诱导独特的巨噬细胞亚群,Frontiers in Immunology

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通过 LPS 和 IL-4 同时刺激诱导独特的巨噬细胞亚群
Frontiers in Immunology ( IF 5.7 ) Pub Date : 2023-04-21 , DOI: 10.3389/fimmu.2023.1111729
Kei Ishida _{1,

2} , Takahiro Nagatake _{1,

3} , Azusa Saika ₁ , Soichiro Kawai ₁ , Eri Node ₁ , Koji Hosomi ₁ , Jun Kunisawa _{1,

2,

4,

5,

6,

7,

8,

9,

10}

Affiliation

Laboratory of Vaccine Materials and Laboratory of Gut Environmental Health, Microbial Research Center for Health and Medicine, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), Ibaraki, Osaka, Japan.
Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan.
Laboratory of Functional Anatomy, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, Kanagawa, Japan.
Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.
Graduate School of Dentistry, Osaka University, Suita, Osaka, Japan.
Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan.
International Vaccine Design Center, Institute of Medical Science, The University of Tokyo, Minato, Tokyo, Japan.
Department of Microbiology and Immunology, Graduate School of Medicine, Kobe University, Kobe, Hyogo, Japan.
Research Organization for Nano and Life Innovation, Waseda University, Shinjuku, Tokyo, Japan.
Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima City, Hiroshima, Japan.

巨噬细胞表现为各种亚型，在各种组织的免疫监视和维持免疫稳态中发挥着多样化且重要的作用。许多体外研究将巨噬细胞分为两大类：脂多糖 (LPS) 诱导的 M1 巨噬细胞和白细胞介素 4 (IL-4) 诱导的 M2 巨噬细胞。但考虑到复杂多样的微环境体内，M1和M2的概念不足以解释巨噬细胞的多样性。在本研究中，我们分析了 LPS 和 IL-4 同时刺激诱导的巨噬细胞（称为 LPS/IL-4 诱导巨噬细胞）的功能。LPS/IL-4 诱导的巨噬细胞是同质群体，显示出 M1 和 M2 巨噬细胞特征的混合。在 LPS/IL-4 诱导的巨噬细胞中，细胞表面 M1 标记物的表达 (IA乙）高于 M1 巨噬细胞，但 iNOS 表达较低，M1 相关基因表达较低（肿瘤坏死因子α和伊尔12p40）与 M1 巨噬细胞中的表达相比有所降低。相反，LPS/IL-4 诱导的巨噬细胞上细胞表面 M2 标记物 CD206 的表达低于 M2 巨噬细胞和 M2 相关基因的表达（精氨酸1,奇3l3，和菲兹1) 多种多样，有精氨酸1大于，菲兹1低于，并且奇3l3与 M2 巨噬细胞中的相似。LPS/IL-4 诱导的巨噬细胞的糖酵解依赖性吞噬活性与 M1 巨噬细胞一样强烈增强；然而，LPS/IL-4诱导的巨噬细胞的能量代谢，如糖酵解和氧化磷酸化的激活状态，与M1或M2巨噬细胞有很大不同。这些结果表明LPS和IL-4诱导的巨噬细胞具有独特的性质。

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Induction of unique macrophage subset by simultaneous stimulation with LPS and IL-4

Macrophages manifest as various subtypes that play diverse and important roles in immunosurveillance and the maintenance of immunological homeostasis in various tissues. Many in vitro studies divide macrophages into two broad groups: M1 macrophages induced by lipopolysaccharide (LPS), and M2 macrophages induced by interleukin 4 (IL-4). However, considering the complex and diverse microenvironment in vivo, the concept of M1 and M2 is not enough to explain diversity of macrophages. In this study, we analyzed the functions of macrophages induced by simultaneous stimulation with LPS and IL-4 (termed LPS/IL-4-induced macrophages). LPS/IL-4-induced macrophages were a homogeneous population showing a mixture of the characteristics of M1 and M2 macrophages. In LPS/IL-4-induced macrophages, expression of cell-surface M1 markers (I-Ab) was higher than in M1 macrophages, but lower expression of iNOS, and expression of M1-associated genes (Tnfα and Il12p40) were decreased in comparison to expression in M1 macrophages. Conversely, expression of the cell-surface M2 marker CD206 was lower on LPS/IL-4-induced macrophages than on M2 macrophages and expression of M2-associated genes (Arg1, Chi3l3, and Fizz1) varied, with Arg1 being greater than, Fizz1 being lower than, and Chi3l3 being comparable to that in M2 macrophages. Glycolysis-dependent phagocytic activity of LPS/IL-4-induced macrophages was strongly enhanced as was that of M1 macrophages; however, the energy metabolism of LPS/IL-4-induced macrophages, such as activation state of glycolytic and oxidative phosphorylation, was quite different from that of M1 or M2 macrophages. These results indicate that the macrophages induced by LPS and IL-4 had unique properties.

更新日期：2023-04-21

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