Radio-Frequency Sweeps at Microtesla Fields for Parahydrogen-Induced Polarization of Biomolecules,The Journal of Physical Chemistry Letters

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Radio-Frequency Sweeps at Microtesla Fields for Parahydrogen-Induced Polarization of Biomolecules
The Journal of Physical Chemistry Letters ( IF 4.8 ) Pub Date : 2023-02-20 , DOI: 10.1021/acs.jpclett.2c03785
Alastair Marshall _{1,

2} , Alon Salhov _{1,

3} , Martin Gierse _{1,

2} , Christoph Müller ₁ , Michael Keim ₁ , Sebastian Lucas ₁ , Anna Parker ₁ , Jochen Scheuer ₁ , Christophoros Vassiliou ₁ , Philipp Neumann ₁ , Fedor Jelezko _{1,

2} , Alex Retzker _{1,

3} , John W Blanchard ₁ , Ilai Schwartz ₁ , Stephan Knecht ₁

Affiliation

Magnetic resonance imaging of ¹³C-labeled metabolites enhanced by parahydrogen-induced polarization (PHIP) enables real-time monitoring of processes within the body. We introduce a robust, easily implementable technique for transferring parahydrogen-derived singlet order into ¹³C magnetization using adiabatic radio frequency sweeps at microtesla fields. We experimentally demonstrate the applicability of this technique to several molecules, including some molecules relevant for metabolic imaging, where we show significant improvements in the achievable polarization, in some cases reaching above 60% nuclear spin polarization. Furthermore, we introduce a site-selective deuteration scheme, where deuterium is included in the coupling network of a pyruvate ester to enhance the efficiency of the polarization transfer. These improvements are enabled by the fact that the transfer protocol avoids relaxation induced by strongly coupled quadrupolar nuclei.

中文翻译：

微特斯拉场的射频扫描用于副氢诱导的生物分子极化

^{通过仲氢诱导极化 (PHIP) 增强的13} C 标记代谢物的磁共振成像能够实时监测体内过程。我们介绍了一种稳健、易于实施的技术，用于将仲氢衍生的单重态转移到¹³在微特斯拉场使用绝热射频扫描的 C 磁化。我们通过实验证明了这种技术对几种分子的适用性，包括一些与代谢成像相关的分子，我们在可实现的极化方面表现出显着改善，在某些情况下达到 60% 以上的核自旋极化。此外，我们引入了一种位点选择性氘化方案，其中氘包含在丙酮酸酯的耦合网络中，以提高极化转移的效率。这些改进是由于传输协议避免了强耦合四极核引起的松弛这一事实而实现的。

更新日期：2023-02-20

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