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Characterization of a novel polysaccharide from Arca subcrenata and its immunoregulatory activities in vitro and in vivo
Food & Function ( IF 5.1 ) Pub Date : 2022-12-31 , DOI: 10.1039/d2fo03483b Hui Shi 1, 2 , Jianhuan Li 1 , Fei Liu 2 , Sixue Bi 1 , Weijuan Huang 3 , Yuanyuan Luo 3 , Man Zhang 1 , Liyan Song 3 , Rongmin Yu 1, 2 , Jianhua Zhu 1, 2
Food & Function ( IF 5.1 ) Pub Date : 2022-12-31 , DOI: 10.1039/d2fo03483b Hui Shi 1, 2 , Jianhuan Li 1 , Fei Liu 2 , Sixue Bi 1 , Weijuan Huang 3 , Yuanyuan Luo 3 , Man Zhang 1 , Liyan Song 3 , Rongmin Yu 1, 2 , Jianhua Zhu 1, 2
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Arca subcrenata is an economical edible shellfish. A novel water-soluble α-D-glucan (ASPG-1) with a molecular weight of 2.56 × 106 Da was purified and characterized from A. subcrenata. Its structure was characterized as a repeating unit consisting of α-D-Glcp, (1 → 6)-α-D-Glcp and (1 → 4,6)-α-D-Glcp. ASPG-1 exerted potent immunoregulatory activity by promoting the viability of splenic lymphocytes. Moreover, it enhanced pinocytic capacity, and promoted the secretion of NO and cytokines in RAW264.7 cells. The immunomodulatory mechanism of ASPG-1 involved the activation of the TLR4-MAPK/Akt-NF-κB signaling pathway. ASPG-1 inhibited tumor growth in 4T1 breast cancer mice and its combination with doxorubicin increased antitumor efficacy. The ASPG-1 combination with DOX-treated group (64.8%) showed an improved tumor inhibition rate compared to that of the DOX-treated group (53.3%). The antitumor mechanism of ASPG-1 may involve an enhancement of the immune response of mice to tumors. These results indicated that ASPG-1 could be developed as a potential adjuvant in tumor immunotherapy.
中文翻译：
Arca subcrenata 一种新型多糖的表征及其体外和体内免疫调节活性
Arca subcrenata是一种经济的食用贝类。从A. subcrenata中纯化并表征了一种分子量为 2.56 × 10 6 Da的新型水溶性 α- D-葡聚糖 (ASPG-1) 。其结构表征为由 α- D -Glc p、(1 → 6)-α- D -Glc p和 (1 → 4,6)-α- D -Glc p组成的重复单元. ASPG-1 通过促进脾淋巴细胞的活力发挥强大的免疫调节活性。此外，它增强胞饮能力，促进RAW264.7细胞分泌NO和细胞因子。ASPG-1 的免疫调节机制涉及激活 TLR4-MAPK/Akt-NF-κB 信号通路。ASPG-1 抑制 4T1 乳腺癌小鼠的肿瘤生长，其与多柔比星的组合增加了抗肿瘤功效。与 DOX 治疗组 (53.3%) 相比，ASPG-1 与 DOX 治疗组 (64.8%) 的组合显示出更高的肿瘤抑制率。ASPG-1的抗肿瘤机制可能与增强小鼠对肿瘤的免疫反应有关。这些结果表明 ASPG-1 可以开发为肿瘤免疫治疗的潜在佐剂。
更新日期：2022-12-31
中文翻译：
Arca subcrenata 一种新型多糖的表征及其体外和体内免疫调节活性
Arca subcrenata是一种经济的食用贝类。从A. subcrenata中纯化并表征了一种分子量为 2.56 × 10 6 Da的新型水溶性 α- D-葡聚糖 (ASPG-1) 。其结构表征为由 α- D -Glc p、(1 → 6)-α- D -Glc p和 (1 → 4,6)-α- D -Glc p组成的重复单元. ASPG-1 通过促进脾淋巴细胞的活力发挥强大的免疫调节活性。此外，它增强胞饮能力，促进RAW264.7细胞分泌NO和细胞因子。ASPG-1 的免疫调节机制涉及激活 TLR4-MAPK/Akt-NF-κB 信号通路。ASPG-1 抑制 4T1 乳腺癌小鼠的肿瘤生长，其与多柔比星的组合增加了抗肿瘤功效。与 DOX 治疗组 (53.3%) 相比，ASPG-1 与 DOX 治疗组 (64.8%) 的组合显示出更高的肿瘤抑制率。ASPG-1的抗肿瘤机制可能与增强小鼠对肿瘤的免疫反应有关。这些结果表明 ASPG-1 可以开发为肿瘤免疫治疗的潜在佐剂。
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