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Biocatalyst collection and heterologous expression of sesquiterpene synthases from basidiomycetous fungi: Discovery of a novel sesquiterpene hydrocarbon
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2022-12-28 , DOI: 10.1111/1751-7915.14204 Natsuki Masunaga 1 , Takuya Kitaoka 1 , Hirofumi Ichinose 1
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2022-12-28 , DOI: 10.1111/1751-7915.14204 Natsuki Masunaga 1 , Takuya Kitaoka 1 , Hirofumi Ichinose 1
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Basidiomycetes produce a wide variety of sesquiterpenoids, which attract significant interest in pharmaceutical and industrial applications. Structural diversification of sesquiterpenoids is performed by sesquiterpene synthases (STSs), which produce a wide array of backbone structures; therefore, functional characterization and increased biocatalyst collection of STSs are important for expanding scientific knowledge and meeting the needs of advanced biotechnology. Gene identification and functional annotation of STSs from the basidiomycetous fungi Agaricus bisporus, Auriscalpium vulgare, Lepista nuda, Pleurotus ostreatus and Trametes versicolor were conducted. Through these investigations, the catalytic functions of 30 STSs were revealed using recombinant enzymes heterologously expressed in Saccharomyces cerevisiae. Furthermore, the unique function of an STS from P. ostreatus, PoSTS-06, was revealed to be the production of a novel sesquiterpene hydrocarbon that we named pleostene. The absolute structure of pleostene was determined by NMR spectroscopy and X-ray crystallography using the crystalline sponge method.
中文翻译：
担子菌倍半萜合酶的生物催化剂收集和异源表达：新型倍半萜烃的发现
担子菌产生多种倍半萜类化合物，在制药和工业应用中引起了极大的兴趣。倍半萜类化合物的结构多样化是通过倍半萜合酶（STS）实现的，它产生多种主链结构；因此，STS 的功能表征和增加生物催化剂收集对于扩展科学知识和满足先进生物技术的需求非常重要。对担子菌类真菌双孢蘑菇、土耳菌、裸丽菌、平菇和栓菌的STS进行了基因鉴定和功能注释。通过这些研究，使用在酿酒酵母中异源表达的重组酶揭示了 30 个 STS 的催化功能。此外， P. ostreatus的 STS （PoSTS-06）的独特功能是产生一种新型倍半萜烃，我们将其命名为 pleostene。使用结晶海绵法通过核磁共振波谱和X射线晶体学测定了新烯的绝对结构。
更新日期：2022-12-28
中文翻译：
担子菌倍半萜合酶的生物催化剂收集和异源表达：新型倍半萜烃的发现
担子菌产生多种倍半萜类化合物，在制药和工业应用中引起了极大的兴趣。倍半萜类化合物的结构多样化是通过倍半萜合酶（STS）实现的，它产生多种主链结构；因此，STS 的功能表征和增加生物催化剂收集对于扩展科学知识和满足先进生物技术的需求非常重要。对担子菌类真菌双孢蘑菇、土耳菌、裸丽菌、平菇和栓菌的STS进行了基因鉴定和功能注释。通过这些研究，使用在酿酒酵母中异源表达的重组酶揭示了 30 个 STS 的催化功能。此外， P. ostreatus的 STS （PoSTS-06）的独特功能是产生一种新型倍半萜烃，我们将其命名为 pleostene。使用结晶海绵法通过核磁共振波谱和X射线晶体学测定了新烯的绝对结构。
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