Tailoring the Hinge Residue at the Substrate Access Tunnel Entrance Improves the Catalytic Performance of Industrialized Nitrile Hydratase Toward 3-Cyanopyridine,ChemistrySelect

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Tailoring the Hinge Residue at the Substrate Access Tunnel Entrance Improves the Catalytic Performance of Industrialized Nitrile Hydratase Toward 3-Cyanopyridine
ChemistrySelect ( IF 1.9 ) Pub Date : 2022-09-09 , DOI: 10.1002/slct.202201941
Yuanyuan Xia ₁ , Meng Yin ₁ , Lukasz Peplowski ₂ , Zhongyi Cheng ₁ , Zhemin Zhou _{1,

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Affiliation

A hinge residue at the entrance of the substrate access tunnel of H-NHase was identified through substrate access tunnel calculations, site-saturation mutagenesis, molecular docking and molecular dynamics simulations. The mutant showed higher specific activity towards 3-cyanopyridine and better thermostability.

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