Compared with three-dimensional bulk MFI zeolites, two-dimensional MFI zeolites have larger surface areas, shorter diffusion distances, and more flexible structures, and they have greater advantages in catalysis. In the synthesis of two-dimensional MFI zeolites, bi-functional templates with hydrophilic and hydrophobic groups play an important role. Templates with different groups and different chain lengths have important effects on the morphologies and catalytic performances of synthesized two-dimensional zeolites. In this study, based on the research status of two-dimensional MFI zeolites in recent years, the synthesis methods, structural modification and cost reduction of layered MFI zeolites are introduced. The advantages and disadvantages of the different synthesis methods are discussed, and the opportunities and challenges in the future are presented.