In this study, the essential oil was extracted and characterized from fresh seed peel waste of Platycladus orientalis (L.) Franco by enzymatic pretreatment combined with solvent-free microwave extraction method (EP-SME) for the first time. The optimal preparation conditions were obtained by single-factor and response surface optimization. Under the optimum conditions, the essential oil yield was 1.13%, which was 24.18% higher than that by traditional steam distillation method (SD) (0.91%), and the essential oil was rich in more polyphenols and flavonoids. The results of gas chromatography-mass spectrometry showed that the essential oil from EP-SME method had the high percentage of oxygenated compound (13.38%). Compared with SD method, the content increments of the first three components were cedrol (+4.91%), 2-carene (+2.31%), caryophyllene (+0.81%), respectively. In addition, the essential oil from the EP-SFME method showed higher comprehensive antioxidant activity and stronger acetylcholinesterase inhibitory activity than those from the hydrodistillation method. The results of extraction kinetics and energy consumption showed that EP-SME method had high extraction efficiency and reached the extraction equilibrium point in a short time (50 min). Its energy consumption and gas emission were only 15.16% of SD.

本研究从侧柏新鲜种子皮废料中提取精油并对其进行表征。(L.) Franco 首次通过酶预处理结合无溶剂微波萃取法 (EP-SME)。通过单因素优化和响应面优化得到最佳制备条件。最佳条件下，精油收率为1.13%，比传统水蒸气蒸馏法（SD）（0.91%）提高24.18%，且精油中多酚类和黄酮类化合物含量较高。气相色谱-质谱分析结果表明，EP-SME法提取的精油含氧化合物含量较高（13.38%）。与SD法相比，前三种成分的含量增量分别为雪松醇（+4.91%）、2-蒈烯（+2.31%）、石竹烯（+0.81%）。此外，EP-SFME法提取的精油比加氢蒸馏法提取的精油具有更高的综合抗氧化活性和更强的乙酰胆碱酯酶抑制活性。提取动力学和能耗结果表明，EP-SME法提取效率高，可在短时间内（50 min）达到提取平衡点。其能耗和气体排放量仅为SD的15.16%。