To enhance the catalytic activity of two-dimensional layered materials as versatile materials, the modification of transition metal dichalcogenide nanosheets such as MoS₂ by doping with heteroatoms has drawn great interests. However, few reports are available on the study of the enzyme-like activity of doped MoS₂. In this study, a facile in situ hydrothermal method for the preparation of various ultrathin transition metals (Fe, Cu, Co, Mn, and Ni) doped MoS₂ nanosheets has been reported. Through the density functional theory (DFT) and steady-state kinetic analysis, the Co-doped MoS₂ nanosheets exhibited the highest peroxidase-like catalytic activity among them. Furthermore, a typical colorimetric assay for H₂O₂ was presented based on the catalytic oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to a blue product (oxTMB) by Co-MoS₂. The proposed colorimetric method showed excellent tolerance under extreme conditions and a broad linear range from 0.0005 to 25 mM for H₂O₂ determination. Concerning the practical application, in situ detection of H₂O₂ generated from SiHa cells was also fulfilled, fully confirming the great practicability of the proposed method in biosensing fields.

为了增强二维层状材料作为通用材料的催化活性，通过掺杂杂原子对过渡金属二硫属化物纳米片如MoS _{2进行改性引起了极大的兴趣。然而，关于掺杂MoS 2}的酶样活性研究的报道很少。本研究报道了一种简便的原位水热法制备各种超薄过渡金属（Fe、Cu、Co、Mn和Ni）掺杂的MoS _{2纳米片。}通过密度泛函理论（DFT）和稳态动力学分析，Co掺杂的MoS ₂纳米片表现出最高的类过氧化物酶催化活性。此外，H _{2的典型比色测定}O ₂是基于 Co-MoS ₂将无色 3,3',5,5'-四甲基联苯胺 (TMB) 催化氧化为蓝色产物 (oxTMB) 而呈现的。_{所提出的比色法在极端条件下表现出优异的耐受性，H 2} O ₂测定的线性范围为 0.0005 至 25 mM 。_{在实际应用方面，还实现了对SiHa细胞产生的H 2} O ₂的原位检测，充分证实了该方法在生物传感领域的巨大实用性。