液相色谱和紫外可见分光光度法测定叶酸抗代谢物 pKa 值的发展,Journal of Pharmaceutical and Biomedical Analysis

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液相色谱和紫外可见分光光度法测定叶酸抗代谢物 pKa 值的发展
Journal of Pharmaceutical and Biomedical Analysis ( IF 3.1 ) Pub Date : 2022-02-10 , DOI: 10.1016/j.jpba.2022.114647
Y Doğan Daldal ₁ , Ebru Çubuk Demiralay ₂

Affiliation

在本研究中，通过反相液相色谱 (RPLC) 和紫外可见分光光度法在 25 °C 下测定了作为叶酸抗代谢物的培美曲塞和雷替曲塞的电离/质子化常数 (pK _{a ) 值。}这 ${}_{s}^{s}{PK}_{一种}$ 通过保留时间 (t _R ) 在乙腈 (ACN) 含量为 13%、15%、17% 和 20% (v/v) 的二元乙腈-水混合物中对 RPLC 方法和 13%、15% 和分光光度法为 17% (v/v)。水性 pK _a值 ( ${}_{w}^{w}{PK}_{一种})$ 研究的化合物的计算从 ${}_{s}^{s}{PK}_{一种}$ 值使用宏观参数（摩尔分数和介电常数），这些参数在溶剂性质中起重要作用。此外，培美曲塞和雷替曲塞的电离度使用以下公式计算： ${}_{w}^{w}{PK}_{一种}$ 价值观。

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Development of liquid chromatographic and UV-visible spectrophotometric methods for determination of pKa values of folic acid antimetabolites

In this study, the values of ionization/protonation constants (pK_a) of pemetrexed and raltitrexed, which are folic acid antimetabolites, were determined by reversed-phase liquid chromatography (RPLC) and UV-visible spectrophotometric methods at 25 °C. The ${}_{s}^{s}{pK}_{a}$ values were evaluated by retention time (t_R) in binary acetonitrile-water mixtures with acetonitrile (ACN) contents of 13%, 15%, 17% and 20% (v/v) for the RPLC method and 13%, 15% and 17% (v/v) for the spectrophotometric method. The aqueous pK_a values ( ${}_{w}^{w}{pK}_{a})$ of the studied compounds were calculated from the ${}_{s}^{s}{pK}_{a}$ value using the macroscopic parameters (mole fraction and dielectric constant), which play an important role in solvent properties. In addition, the degree of ionization of pemetrexed and raltitrexed was calculated using the ${}_{w}^{w}{pK}_{a}$ values.

更新日期：2022-02-10

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