Surface-Enhanced Raman Scattering Identification of Nucleic Acid Targets by Acetylene-Tagged Hoechst Molecule Binding with DNA-Tethered Gold Nanoparticles,ACS Applied Nano Materials

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Surface-Enhanced Raman Scattering Identification of Nucleic Acid Targets by Acetylene-Tagged Hoechst Molecule Binding with DNA-Tethered Gold Nanoparticles
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2022-02-07 , DOI: 10.1021/acsanm.2c00213
Hiroki Makanai ₁ , Tatsuya Nishihara ₁ , Kazuhito Tanabe ₁

Affiliation

Raman probes have been utilized to identify biomolecules. However, their application for nucleic acid sensing remains limited because of the requirement of purification processes. Here, we provide a general purification-free method for the detection of specific nucleic acids based on surface-enhanced Raman scattering (SERS) using functionalized gold nanoparticles. We identified the robust Raman signal of an acetylene-tagged Hoechst molecule (AH) through SERS because of its binding to nanoparticles bearing double-stranded oligodeoxynucleotides on their surface. Once target nucleic acids were added to the system, strand displacement on the particles and dissociation of AH from the particles occurred spontaneously, leading to a dramatic decrease in the signal intensity. We also found that the cellular miRNA level was successfully identified by the system.

中文翻译：

乙炔标记的赫斯特分子与 DNA 束缚金纳米粒子结合的表面增强拉曼散射鉴定核酸靶点

拉曼探针已被用于识别生物分子。然而，由于纯化过程的要求，它们在核酸传感方面的应用仍然受到限制。在这里，我们提供了一种通用的免纯化方法，用于基于表面增强拉曼散射 (SERS) 使用功能化金纳米粒子检测特定核酸。我们通过 SERS 鉴定了乙炔标记的赫斯特分子 (AH) 的强大拉曼信号，因为它与表面上带有双链寡脱氧核苷酸的纳米颗粒结合。一旦将目标核酸添加到系统中，粒子上的链位移和 AH 从粒子的解离自发发生，导致信号强度急剧下降。

更新日期：2022-02-07

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