线粒体依赖性氧化应激介导 ZnO 纳米颗粒 (ZnO NP) 诱导的淡水硬骨鱼线粒体自噬和脂毒性,Environmental Science & Technology

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线粒体依赖性氧化应激介导 ZnO 纳米颗粒 (ZnO NP) 诱导的淡水硬骨鱼线粒体自噬和脂毒性
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2022-02-02 , DOI: 10.1021/acs.est.1c07198
Guang-Hui Chen ₁ , Chang-Chun Song ₁ , Tao Zhao ₁ , Christer Hogstrand ₂ , Xiao-Lei Wei ₁ , Wu-Hong Lv ₁ , Yu-Feng Song ₁ , Zhi Luo _{1,

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Affiliation

由于许多特殊的特性，氧化锌纳米粒子（ZnO NPs）在世界范围内被广泛使用，导致它们在环境中的广泛分布。然而，环境 ZnO NP 引起的生理过程和代谢变化的毒性和机制仍然很大程度上未知。在这里，我们发现添加 ZnO NPs 会扰乱肝脏 Zn 代谢，增加肝脏 Zn 和脂质积累，下调脂肪分解，诱导氧化应激和激活线粒体自噬；N , N , N ', N '-四(2-吡啶基甲基)乙二胺(TPEN, Zn ²⁺离子螯合剂）减轻了高 ZnO NP 诱导的 Zn 和脂质积累、氧化应激和线粒体自噬。从机制上讲，线粒体氧化应激的抑制减弱了 ZnO NP 激活的线粒体自噬和 ZnO NP 诱导的脂毒性。总之，我们的研究阐明了线粒体氧化应激介导了 ZnO NP 诱导的线粒体自噬和脂毒性。ZnO NPs 可以解离成游离的 Zn ²⁺离子，这部分地促成了 ZnO NP 诱导的氧化应激、线粒体自噬和脂质代谢的变化。我们的研究为 ZnO NPs 作为有害物质诱导水生生物脂毒性的影响和机制提供了新的见解，因此，代谢相关参数将有助于环境中纳米粒子材料的风险评估。

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Mitochondria-Dependent Oxidative Stress Mediates ZnO Nanoparticle (ZnO NP)-Induced Mitophagy and Lipotoxicity in Freshwater Teleost Fish

Due to many special characteristics, zinc oxide nanoparticles (ZnO NPs) are widely used all over the world, leading to their wide distribution in the environment. However, the toxicities and mechanisms of environmental ZnO NP-induced changes of physiological processes and metabolism remain largely unknown. Here, we found that addition of dietary ZnO NPs disturbed hepatic Zn metabolism, increased hepatic Zn and lipid accumulation, downregulated lipolysis, induced oxidative stress, and activated mitophagy; N,N,N′,N′-tetrakis (2-pyridylmethyl) ethylenediamine (TPEN, Zn²⁺ ions chelator) alleviated high ZnO NP-induced Zn and lipid accumulation, oxidative stress, and mitophagy. Mechanistically, the suppression of mitochondrial oxidative stress attenuated ZnO NP-activated mitophagy and ZnO NP-induced lipotoxicity. Taken together, our study elucidated that mitochondrial oxidative stress mediated ZnO NP-induced mitophagy and lipotoxicity; ZnO NPs could be dissociated to free Zn²⁺ ions, which partially contributed to ZnO NP-induced changes in oxidative stress, mitophagy, and lipid metabolism. Our study provides novel insights into the impacts and mechanism of ZnO NPs as harmful substances inducing lipotoxicity of aquatic organisms, and accordingly, metabolism-relevant parameters will be useful for the risk assessment of nanoparticle materials in the environment.

更新日期：2022-02-02

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