Yarrowia lipolytica is recognized as an excellent non-conventional yeast in the field of biomanufacturing, where it is used as a host to produce oleochemicals, terpenes, organic acids, polyols and recombinant proteins. Consequently, metabolic engineering of this yeast is becoming increasingly popular to advance it as a superior biomanufacturing platform, of which promoters are the most basic elements for tuning gene expression. Endogenous promoters of Yarrowia lipolytica were reviewed, which are the basis for promoter engineering. The engineering strategies, such as hybrid promoter engineering, intron enhancement promoter engineering, and transcription factor-based inducible promoter engineering are described. Additionally, the applications of Yarrowia lipolytica promoter engineering to rationally reconstruct biosynthetic gene clusters and improve the genome-editing efficiency of the CRISPR-Cas systems were reviewed. Finally, research needs and future directions for promoter engineering are also discussed in this review.

解脂耶氏酵母在生物制造领域被公认为一种优秀的非常规酵母，它被用作生产油脂化学品、萜烯、有机酸、多元醇和重组蛋白的宿主。因此，这种酵母的代谢工程正变得越来越流行，以将其作为一个优越的生物制造平台，其中启动子是调节基因表达的最基本元素。对解脂耶氏酵母的内源启动子进行了综述，这些启动子是启动子工程的基础。描述了工程策略，例如混合启动子工程、内含子增强启动子工程和基于转录因子的诱导型启动子工程。此外，解脂耶氏酵母的应用综述了合理重建生物合成基因簇和提高CRISPR-Cas系统基因组编辑效率的启动子工程。最后，本文还讨论了启动子工程的研究需求和未来方向。