Cross-Linking Mass Spectrometry for Investigating Protein Conformations and Protein–Protein Interactions─A Method for All Seasons,Chemical Reviews

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Cross-Linking Mass Spectrometry for Investigating Protein Conformations and Protein–Protein Interactions─A Method for All Seasons
Chemical Reviews ( IF 51.4 ) Pub Date : 2021-11-19 , DOI: 10.1021/acs.chemrev.1c00786
Lolita Piersimoni _{1,

2} , Panagiotis L Kastritis _{3,

4,

5} , Christian Arlt _{1,

2} , Andrea Sinz _{1,

2}

Affiliation

Mass spectrometry (MS) has become one of the key technologies of structural biology. In this review, the contributions of chemical cross-linking combined with mass spectrometry (XL-MS) for studying three-dimensional structures of proteins and for investigating protein–protein interactions are outlined. We summarize the most important cross-linking reagents, software tools, and XL-MS workflows and highlight prominent examples for characterizing proteins, their assemblies, and interaction networks in vitro and in vivo. Computational modeling plays a crucial role in deriving 3D-structural information from XL-MS data. Integrating XL-MS with other techniques of structural biology, such as cryo-electron microscopy, has been successful in addressing biological questions that to date could not be answered. XL-MS is therefore expected to play an increasingly important role in structural biology in the future.

中文翻译：

用于研究蛋白质构象和蛋白质-蛋白质相互作用的交联质谱——一种四季皆宜的方法

质谱（MS）已成为结构生物学的关键技术之一。在这篇综述中，概述了化学交联结合质谱 (XL-MS) 在研究蛋白质的三维结构和研究蛋白质-蛋白质相互作用方面的贡献。我们总结了最重要的交联试剂、软件工具和 XL-MS 工作流程，并重点介绍了表征蛋白质、它们的组装和体外和体内相互作用网络的突出示例. 计算建模在从 XL-MS 数据中获取 3D 结构信息方面起着至关重要的作用。将 XL-MS 与其他结构生物学技术（如低温电子显微镜）相结合，已成功解决了迄今为止无法回答的生物学问题。因此，XL-MS 有望在未来在结构生物学中发挥越来越重要的作用。

更新日期：2021-11-19

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