A series of β-Bi₂O₃ with different Co doping amounts were prepared by a co-precipitation and pyrolysis process. A flower-like self-assembly of β-Bi₂O₃ sheets was realized by the doping of Co. Simulation results showed that an impurity state was introduced into the band gap of doped β-Bi₂O₃, mainly due to the Co 3d orbital. Co doping enhanced the visible light utilization rate by reducing the band gap and improving electrons holes separation efficiency. E. coli was used to evaluate the photocatalytic antibacterial ability of undoped and Co-doped β-Bi₂O₃ under visible light. β-Bi₂O₃ exhibited optimal efficiency for both electron-hole separation and antibacterial effect when doped with 0.4% Co (0.4%-CBO). Gradient experiments were performed to determine that reactive oxygen species (ROS) were the main factor behind the antibacterial effect of β-Bi₂O₃. The enhanced antibacterial properties of 0.4%-CBO were due to its increased production of O₂^–, OH, and H₂O₂, with scavenger experiments finding that O₂^– was the main contributor to its antibacterial effect. Through the detection of lipid peroxidation and bacterial respiratory chain dehydrogenase activity, it was determined that the antibacterial effect of ROS simultaneously occurred in the interior and exterior of E. coli and that this activity was enhanced by Co doping.

通过共沉淀和热解工艺制备了一系列具有不同Co掺杂量的β-Bi ₂ O ₃。Co的掺杂实现了β-Bi ₂ O ₃片层的花状自组装。模拟结果表明，掺杂的β-Bi ₂ O ₃的带隙中引入了杂质态，主要是由于Co 3d 轨道。Co掺杂通过减小带隙和提高电子空穴分离效率来提高可见光利用率。大肠杆菌被用来评估未掺杂和Co掺杂的β-Bi ₂ O ₃在可见光下的光催化抗菌能力。β-Bi ₂ O当掺杂 0.4% Co (0.4%-CBO) 时，₃表现出最佳的电子-空穴分离效率和抗菌效果。进行梯度实验以确定活性氧 (ROS) 是 β-Bi ₂ O ₃抗菌作用背后的主要因素。0.4%-CBO 增强的抗菌性能是由于其增加了O ₂ ^–、OH 和 H ₂ O _{2 的产生}，清除剂实验发现O ₂ ^–是其抗菌作用的主要贡献者。通过脂质过氧化和细菌呼吸链脱氢酶活性的检测，确定ROS的抗菌作用在大肠杆菌的内部和外部同时发生，并且这种活性通过Co掺杂增强。