Analytical and Bioanalytical Chemistry ( IF 3.8 ) Pub Date : 2021-08-26 , DOI: 10.1007/s00216-021-03624-w Shuting Xu 1, 2 , Cheng Yang 1, 2 , Xiuping Yan 1, 2 , Huwei Liu 3
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Mass spectrometry (MS) is attractive for single-cell analysis because of its high sensitivity, rich information, and large dynamic ranges, especially for the single-cell metabolome and proteome analysis. Efforts have been made to deal with the throughput and information coverage problems in typical manual single-cell MS techniques. In this review, advanced techniques to improve the automation and throughput for single-cell sampling and single-cell metabolome and proteome MS detection have been discussed. Furthermore, representative MS-based strategies that can increase the in-depth cellular information coverage and achieve the more comprehensive single-cell multiomics information during high throughput detection have been highlighted, providing an ongoing perspective of the MS performance for the single-cell research.
Graphical abstract
中文翻译：
迈向高通量和高信息覆盖：先进的单细胞质谱技术
质谱 (MS) 因其高灵敏度、丰富的信息和大的动态范围而对单细胞分析具有吸引力，特别是对于单细胞代谢组和蛋白质组分析。已经努力处理典型手动单小区MS技术中的吞吐量和信息覆盖问题。在这篇综述中，讨论了提高单细胞采样和单细胞代谢组和蛋白质组 MS 检测的自动化和通量的先进技术。此外，还强调了具有代表性的基于 MS 的策略，这些策略可以增加深入的蜂窝信息覆盖，并在高通量检测期间获得更全面的单细胞多组学信息，为单细胞研究的 MS 性能提供了一个持续的视角。
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