Amphiphilic graphene quantum dots (A-GQDs) reveal prodigious potential application in bioimaging, resulting from their hydrophilic and lipophilic properties, as well as their good biocompatibility and negligible cytotoxicity. Herein, green fluorescent A-GQDs with single-layer graphene structure were prepared by one step of hydrothermal method. The A-GQDs with the average size of 3.5 nm displayed excitation-independent emission due to uniform size. A-GQDs emitted bright fluorescence on the surface of the cell membrane of MCF-7 cells when they were co-cultured with MCF-7 cells, which was similar to that of QSG-7701 cells. The water-soluble pillar[5]arene (WP5) was then incorporated on to the A-GQDs via π-π interaction and electrostatic interaction. There was different labeling imaging effect of MCF-7 and QSG-7701 cells achieved by combining the merits of WP5 and A-GQDs compared with individual A-GQDs. The synthesized composites not only had a certain enhancement in fluorescence, but also constructed a penetrating channel through the cell membrane, so that the entire cell had a distinct imaging. Therefore, these composites might be a potential candidate for applications in visualization of cell membrane and bioimaging.

两亲性石墨烯量子点（A-GQD）由于其亲水性和亲脂性，以及良好的生物相容性和可忽略的细胞毒性，在生物成像中显示出巨大的潜在应用。在此，通过一步水热法制备了具有单层石墨烯结构的绿色荧光A-GQDs。由于尺寸均匀，平均尺寸为 3.5 nm 的 A-GQD 显示出与激发无关的发射。A-GQDs与MCF-7细胞共培养时，MCF-7细胞的细胞膜表面发出明亮的荧光，与QSG-7701细胞相似。然后通过π-π相互作用和静电相互作用将水溶性柱[5]芳烃（WP5）结合到A-GQD上。与单独的A-GQDs相比，结合WP5和A-GQDs的优点，MCF-7和QSG-7701细胞的标记成像效果不同。合成的复合材料不仅具有一定的荧光增强作用，而且还构建了贯穿细胞膜的通道，使整个细胞具有明显的成像效果。因此，这些复合材料可能是细胞膜可视化和生物成像应用的潜在候选者。