A Class II CPD Photolyase and a 6-4 Photolyase with Photorepair Activity from the Antarctic Moss Pohlia nutans M211,Photochemistry and Photobiology

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A Class II CPD Photolyase and a 6-4 Photolyase with Photorepair Activity from the Antarctic Moss Pohlia nutans M211
Photochemistry and Photobiology ( IF 2.6 ) Pub Date : 2021-06-24 , DOI: 10.1111/php.13478
Meiling An _{1,

2} , Changfeng Qu _{2,

3} , Jinlai Miao _{2,

3} , Zhenxia Sha ₁

Affiliation

Antarctic mosses are the dominant vegetation in the Antarctic continent. Because of stratospheric ozone depletion, they can withstand physiological extreme UV. The formation of CPD and 6-4PP is one of the most harmful damages of UV to DNA. DNA damage can interfere with replication and transcription, resulting in mutation and death. Two types of photolyase, CPD photolyase and 6-4 photolyase, are capable of specific binding CPD or 6-4PP and repairing these lesions. However, there is little research on photolyase in Antarctic moss. Here, we isolated a gene encoding class II CPD photolyase (PnCPDPhr) and a gene encoding 6-4 photolyase (Pn6-4Phr) from Antarctic moss P. nutans M211. When exposed to UVB, CPDs accumulated in gametophytes and the gene expressions of PnCPDPhr and Pn6-4Phr were both up-regulated. In addition, the in vitro expression and photoreactivation assays of PnCPDPhr and Pn6-4Phr were performed. Our results demonstrated that PnCPDPhr and Pn6-4Phr have an effective activity of DNA repair. This is the first study to determine the CPD accumulation in Antarctic moss as well as the first report isolating CPD photolyase and 6-4 photolyase from Antarctic moss. These results will enrich the knowledge of photolyase family and benefit the exploitation of functioning gene in Antarctic moss.

中文翻译：

来自南极苔藓 Pohlia nutans M211 的 II 类 CPD 光解酶和具有光修复活性的 6-4 光解酶

南极苔藓是南极大陆的主要植被。由于平流层臭氧消耗，它们可以承受生理极端紫外线。CPD和6-4PP的形成是UV对DNA最有害的损伤之一。DNA 损伤会干扰复制和转录，导致突变和死亡。两种类型的光解酶CPD光解酶和6-4光解酶能够特异性结合CPD或6-4PP并修复这些病变。然而，关于南极苔藓中的光解酶的研究很少。在这里，我们从南极苔藓P. nutans M211 中分离出一个编码 II 类 CPD 光解酶 ( PnCPDPhr ) 的基因和一个编码 6-4 光解酶 ( Pn6-4Phr ) 的基因。当暴露于 UVB 时，CPDs 在配子体中积累和基因表达PnCPDPhr和Pn6-4Phr均上调。此外，还进行了 PnCPDPhr 和 Pn6-4Phr的体外表达和光活化测定。我们的研究结果表明，PnCPDPhr 和 Pn6-4Phr 具有有效的 DNA 修复活性。这是第一个确定南极苔藓中CPD积累的研究，也是第一个从南极苔藓中分离出CPD光解酶和6-4光解酶的报告。这些结果将丰富光解酶家族的知识，有利于南极苔藓功能基因的开发。

更新日期：2021-06-24

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