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Identification and characterization of microRNAs in the immature stage of the beneficial predatory bug Arma chinensis Fallou (Hemiptera: Pentatomidae)
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2021-06-01 , DOI: 10.1002/arch.21796 Yanfang Yin 1 , Yanjuan Zhu 1 , Jianjun Mao 1 , Dawn E Gundersen-Rindal 2 , Chenxi Liu 1
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2021-06-01 , DOI: 10.1002/arch.21796 Yanfang Yin 1 , Yanjuan Zhu 1 , Jianjun Mao 1 , Dawn E Gundersen-Rindal 2 , Chenxi Liu 1
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MicroRNAs (miRNAs) are a type of small noncoding RNAs that regulate gene expression at the posttranscriptional level and can influence significant biological processes. Arma chinensis (Hemiptera: Pentatomidae) is a predaceous insect species that preys upon a wide variety of insect pests. It is important to explore and understand the molecular mechanisms involving miRNAs in regulating developmental and other gene expression for beneficial insects. However, examination of miRNAs associated with Hemiptera, especially predatory bugs, has been absent or scarce. This study represents the first comprehensive analysis of predatory bug A. chinensis encoded miRNAs through high throughput sequencing and predicts genes and biological processes regulated by the newly identified miRNAs through analyzing their differential expression in and across five nymphal instars. A total of 64 A. chinensis miRNAs, including 46 conserved miRNAs and 18 novel miRNAs, were identified by analysis of high throughput sequence reads mapped to the genome. A total of 2913 potential gene targets for these 64 miRNAs were predicted by comprehensive analyses utilizing miRanda, PITA, and RNAhybrid. Gene Ontology annotation of predicted target genes of A. chinensis suggested the key processes regulated by miRNAs involved biological processes, regulation of cellular processes, and transporter activity. Kyoto Encyclopedia of Genes and Genomes pathway predictions included the Toll and Imd signaling pathway, Valine, leucine and isoleucine degradation, Steroid biosynthesis, the AGE-RAGE signaling pathway in diabetic complications, and Alanine, aspartate and glutamate metabolism. This newly identified miRNAs through analyzing their differential expression, assessment of their predicted functions forms a foundation for further investigation of specific miRNAs.
中文翻译：
有益掠食性昆虫 Arma chinensis Fallou（半翅目：五足科）未成熟阶段 microRNA 的鉴定和表征
MicroRNAs (miRNAs) 是一类小的非编码 RNA，在转录后水平调节基因表达，可以影响重要的生物过程。Arma chinensis (Hemiptera: Pentatomidae) 是一种捕食性昆虫，以多种害虫为食。探索和理解涉及 miRNA 调节益虫发育和其他基因表达的分子机制非常重要。然而，与半翅目相关的 miRNA 的检查，尤其是捕食性昆虫，一直缺乏或很少。这项研究代表了对掠食性昆虫A. chinensis的首次综合分析通过高通量测序编码 miRNA，并通过分析它们在五个若虫龄内和之间的差异表达来预测由新发现的 miRNA 调控的基因和生物过程。通过对映射到基因组的高通量序列读数的分析，确定了总共 64 种A. chinensis miRNA，包括 46 个保守的 miRNA 和 18 个新的 miRNA。通过利用 miRanda、PITA 和 RNAhybrid 的综合分析，预测了这 64 个 miRNA 的总共 2913 个潜在基因靶点。的预测靶基因的基因本体论诠释中华猕猴桃表明 miRNA 调控的关键过程涉及生物过程、细胞过程的调节和转运蛋白活性。京都基因和基因组百科全书通路预测包括 Toll 和 Imd 信号通路、缬氨酸、亮氨酸和异亮氨酸降解、类固醇生物合成、糖尿病并发症中的 AGE-RAGE 信号通路以及丙氨酸、天冬氨酸和谷氨酸代谢。这种通过分析其差异表达、评估其预测功能而新发现的 miRNA 为进一步研究特定 miRNA 奠定了基础。
更新日期：2021-06-23
中文翻译：
有益掠食性昆虫 Arma chinensis Fallou（半翅目：五足科）未成熟阶段 microRNA 的鉴定和表征
MicroRNAs (miRNAs) 是一类小的非编码 RNA，在转录后水平调节基因表达，可以影响重要的生物过程。Arma chinensis (Hemiptera: Pentatomidae) 是一种捕食性昆虫，以多种害虫为食。探索和理解涉及 miRNA 调节益虫发育和其他基因表达的分子机制非常重要。然而，与半翅目相关的 miRNA 的检查，尤其是捕食性昆虫，一直缺乏或很少。这项研究代表了对掠食性昆虫A. chinensis的首次综合分析通过高通量测序编码 miRNA，并通过分析它们在五个若虫龄内和之间的差异表达来预测由新发现的 miRNA 调控的基因和生物过程。通过对映射到基因组的高通量序列读数的分析，确定了总共 64 种A. chinensis miRNA，包括 46 个保守的 miRNA 和 18 个新的 miRNA。通过利用 miRanda、PITA 和 RNAhybrid 的综合分析，预测了这 64 个 miRNA 的总共 2913 个潜在基因靶点。的预测靶基因的基因本体论诠释中华猕猴桃表明 miRNA 调控的关键过程涉及生物过程、细胞过程的调节和转运蛋白活性。京都基因和基因组百科全书通路预测包括 Toll 和 Imd 信号通路、缬氨酸、亮氨酸和异亮氨酸降解、类固醇生物合成、糖尿病并发症中的 AGE-RAGE 信号通路以及丙氨酸、天冬氨酸和谷氨酸代谢。这种通过分析其差异表达、评估其预测功能而新发现的 miRNA 为进一步研究特定 miRNA 奠定了基础。
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