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Diversity in capsule and seed morphological and phytochemical features and essential oil composition of korarima (Aframomum corrorima (braun) P. C. M. jansen) collections from Ethiopia
Biochemical Systematics and Ecology ( IF 1.4 ) Pub Date : 2021-05-17 , DOI: 10.1016/j.bse.2021.104275
Tilahun Hailu , Tileye Feyissa , Aman Dekebo , Girma Hailemichael , Fekadu Gadissa

Korarima (Aframomum corrorima (Braun) P. C. M. Jansen) is one of the Ethiopian native perennial aromatic herbs belonging to the family Zingiberaceae. Conservation of genotypes and genetic improvement of korarima has been hampered due to lack of evidences on genetic diversity of korarima. Genetic diversity of 44 korarima collections was assessed based on joint capsules and seeds morphological and phytochemical characters. Chemical composition of essential oil in seed samples has also been investigated. Physical measurements were done manually while total organic nutrients, crude fiber and total ash in the seeds were done in terms of proximate analysis. Essential oil in the seed samples was extracted by hydro-distillation and its chemical composition was analyzed with gas chromatography and mass spectrometry (GC-MS). ANOVA revealed significant variations in all the variables considered except capsule and seed shape. Ayda collections showed the largest record for fresh capsule weight (25.3 g), dry capsule weight (6.6 g), hundred seed weight (2.8 g), seed to husk ratio (2.1), total organic matter (66.6%) and seed essential oil (7.3%). Collection from Bazet was also excellent in dry weight of seed per capsule (4.45 g) while those from Metser were best in fruit husk essential oil (1.45%). Cluster analysis of the 44 collections revealed four groups each showing large (23.82–280.13) and significant (p < 0.01) pairwise genetic distance. The pattern of grouping is weak and does not match with the region of collections indicating the presence of different genetic background within the collections as well as genetic similarity among the collections. Principal component analysis (PCA) revealed about 81.2% of the total variations in the first four PCs and the pattern of grouping is similar to cluster analysis. In Essential oil composition, Bona Kike collection was remarkable in 1, 8-Cineole (60.81%). Ayda collection showed the highest m-Mentha-1,8-diene, (+)- (10.05%) and β-farnesene (24.03%). Adele Bise collection was highest in D-Limonene (20.21%). The genetic variability in essential oil composition among the samples was revealed because of spatial separation. The result suggests the potential of Ethiopian korarima in general and the collections in particular for further improvement and commercialization. Overall, the higher extent of morphological and biochemical genetic diversity observed signpost the high opportunity for genetic improvement of the crop via hybridization and selection.



中文翻译:

埃塞俄比亚korarima(Aframomum corrorima(braun)PCM jansen)的荚膜和种子形态,植物化学特征和精油成分的多样性

科拉里玛(Aframomum corrorima)(Braun)PCM Jansen)是属于姜科的埃塞俄比亚本地多年生芳香植物之一。由于缺乏有关小核糖遗传多样性的证据,阻碍了小核糖的基因型保存和遗传改良。基于联合荚膜和种子的形态和植物化学特征,评估了44种小菜豆的遗传多样性。还对种子样品中精油的化学成分进行了研究。物理测量是手动完成的,而种子中的总有机养分,粗纤维和总灰分则是根据近距离分析完成的。通过水蒸馏法提取种子样品中的香精油,并通过气相色谱和质谱(GC-MS)分析其化学成分。方差分析显示,除荚膜和种子形状外,所有其他变量均存在显着差异。艾达(Ayda)采集的鲜荚重(25.3 g),干荚重(6.6 g),百粒重(2.8 g),籽壳比(2.1),总有机质(66.6%)和种子精油的最高记录(7.3%)。从巴兹特收集的每粒种子的干重也极好(4.45 g),而从迈索收集的则以果皮精油最好(1.45%)。对44个馆藏的聚类分析显示,四组分别显示较大的(23.82–280.13)和显着的(p <0.01)成对遗传距离。分组的模式很弱,并且与集合区域不匹配,表明集合内存在不同的遗传背景以及集合之间的遗传相似性。主成分分析(PCA)显示前四台PC中约有81.2%的总变异,并且分组的模式类似于聚类分析。在精油成分中,Bona Kike的收集量在1、8-Cineole(60.81%)中非常出色。Ayda收集物显示最高的m-Mentha-1,8-二烯,(+)-(10.05%)和β-法呢烯(24.03%)。阿黛尔·比塞(Adele Bise)的D-柠檬烯含量最高(20.21%)。由于空间分离,揭示了样品之间精油成分的遗传变异性。结果表明,总体而言,埃塞俄比亚科拉里马具有潜力,特别是其收藏品有进一步改进和商业化的潜力。总体而言,观察到的形态和生化遗传多样性程度更高,预示着作物遗传改良的高机会 前四台PC的总变化的2%和分组方式与聚类分析相似。在精油成分中,Bona Kike的收集量在1、8-Cineole(60.81%)中非常出色。Ayda收集物显示最高的m-Mentha-1,8-二烯,(+)-(10.05%)和β-法呢烯(24.03%)。阿黛尔·比塞(Adele Bise)的D-柠檬烯含量最高(20.21%)。由于空间分离,揭示了样品之间精油成分的遗传变异性。结果表明,总体而言,埃塞俄比亚科拉里马具有潜力,特别是其收藏品有进一步改进和商业化的潜力。总体而言,观察到的形态和生化遗传多样性程度更高,预示着作物遗传改良的高机会 前四台PC的总变化的2%和分组方式与聚类分析相似。在精油成分中,Bona Kike的收集量在1、8-Cineole(60.81%)中非常出色。Ayda收集物显示最高的m-Mentha-1,8-二烯,(+)-(10.05%)和β-法呢烯(24.03%)。阿黛尔·比塞(Adele Bise)的D-柠檬烯含量最高(20.21%)。由于空间分离,揭示了样品之间精油成分的遗传变异性。结果表明,总体而言,埃塞俄比亚科拉里马具有潜力,特别是其收藏品有进一步改进和商业化的潜力。总体而言,观察到的形态和生化遗传多样性程度更高,预示着作物遗传改良的高机会通过杂交和选择。

更新日期:2021-05-18
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