超声波和微波辅助提取菠萝副产品中菠萝蛋白酶和生物活性肽的比较提取,Journal of Food Process Engineering

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超声波和微波辅助提取菠萝副产品中菠萝蛋白酶和生物活性肽的比较提取
Journal of Food Process Engineering ( IF 2.7 ) Pub Date : 2021-04-23 , DOI: 10.1111/jfpe.13709
Thatchajaree Mala ₁ , Muhammad Bilal Sadiq ₂ , Anil Kumar Anal ₁

Affiliation

超声波辅助提取 (UAE) 和微波辅助提取 (MAE) 用于从菠萝副产品中提取生物活性肽。阿联酋的独立提取参数是超声振幅 (60-100%)、提取时间 (10-30 分钟) 和溶剂与材料的比率 (20-40 mL/g)，而 MAE 的独立变量是微波功率 (100-300 W)、辐照时间 (5–15 分钟) 和进料与溶剂比 (1:8–1:12 g/mL)。优化的萃取条件对于UAE 是99.96%、26.83 分钟和20.96 mL/g，对于MAE 是100 W、8.99 分钟和1:8 g/mL。优化的提取导致总糖、蛋白质含量和蛋白水解活性分别为 15.71 ± 0.03 mg/mL、4.01 ± 0.04 mg/mL 和 196.46 ± 3.29 U/mL，而 33.87 ± 0.03 mg/mL，2.50 ± 0.01 MAE 分别为 mg/mL 和 154.08 ± 1.49 U/mL。提取过程中采用扫描电子显微镜观察样品的形貌。分子量测定表明提取物（UAE 和 MAE）中的主要蛋白质条带在~23 kDa 处，对应于菠萝蛋白酶。此外，UAE 获得的菠萝冠蛋白提取物显示出高蛋白水解活性（>80% 相对）。目前的研究表明，菠萝副产品中的菠萝蛋白酶和其他生物活性肽在食品、饲料和医药产品开发方面具有潜力。

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Comparative extraction of bromelain and bioactive peptides from pineapple byproducts by ultrasonic- and microwave-assisted extractions

Ultrasonic-assisted extraction (UAE) and microwave-assisted extraction (MAE) were used for the extraction of bioactive peptides from pineapple byproducts. Independent extraction parameters for UAE were ultrasonic amplitude (60–100%), extraction time (10–30 min), and solvent to material ratio (20–40 mL/g), whereas for MAE independent variables were microwave power (100–300 W), irradiation time (5–15 min), and feed to solvent ratio (1:8–1:12 g/mL). The optimized extraction conditions were 99.96%, 26.83 min, and 20.96 mL/g for UAE and 100 W, 8.99 min, and 1:8 g/mL for MAE. The optimized extraction resulted in total sugar, protein content, and proteolytic activity of 15.71 ± 0.03 mg/mL, 4.01 ± 0.04 mg/mL, and 196.46 ± 3.29 U/mL, respectively, whereas 33.87 ± 0.03 mg/mL, 2.50 ± 0.01 mg/mL, and 154.08 ± 1.49 U/mL for MAE, respectively. Scanning electron microscopy was used to observe the morphology of sample during extraction. Molecular weight determination showed that the major protein band in the extracts (UAE and MAE) was at ∼23 kDa which corresponded to bromelain. Furthermore, pineapple crown protein extract obtained by UAE showed high proteolytic activity (>80% relative). The present study indicated that bromelain and other bioactive peptides from pineapple byproducts have potential in food, feed, and pharmaceutical products development.

更新日期：2021-06-01

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