Echinops kebericho M. (Asteraceae) 80% 甲醇根提取物和衍生溶剂组分在小鼠模型中解热和抗氧化活性的药理学评价,BioMed Research International

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Echinops kebericho M. (Asteraceae) 80% 甲醇根提取物和衍生溶剂组分在小鼠模型中解热和抗氧化活性的药理学评价
BioMed Research International ( IF 2.6 ) Pub Date : 2021-03-16 , DOI: 10.1155/2021/6670984
Tesfaye Yimer ₁ , Yohannes Kelifa Emiru ₂ , Zemene Demelash Kifle ₃ , Amien Ewunetei ₁ , Meaza Adugna ₃ , Eshetie Melese Birru ₃

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背景。在大多数标准药物（包括退热剂）中，毒性和不良影响是非常明显的。迄今为止，从传统植物中寻找毒性和副作用最小的可想象的解热药是一个日益受到关注的问题。Echinops keberico M.（菊科）是最着名的传统药用植物之一，埃塞俄比亚民间医学中的传统治疗师经常证明其传统上声称可用于治疗发烧和不同的感染性和非感染性疾病。然而，这种植物的传统用途尚未得到科学评估。因此，本研究旨在研究 80% 甲醇根提取物及其衍生溶剂馏分的解热和抗氧化活性。小鼠模型中的Echinops keberico M.。方法. 提取方法采用极性增加的连续溶剂浸渍法，溶剂采用氯仿、乙酸乙酯、甲醇和水。提取后，使用酵母诱导的发热评估粗提物及其衍生溶剂级分的解热活性，同时使用二苯基-2-苦基肼（DPPH）测定法在体外测量抗氧化活性。在 100、200 和 400 mg/kg 的剂量下评估提取物和溶剂部分的解热活性，并在 50、100、200、400、600、800 和 1000 的剂量下评估抗氧化活性毫克/公斤。阳性对照组给予标准药物（ASA 100 mg/kg），生理盐水组作为阴性对照组。结果。E。 _ keberico粗提物及其衍生的溶剂馏分显示出统计学意义 (, 0.01 和 0.001) 降温活动。通过粗提物的最高剂量 (400 mg/kg) 观察到温度降低的最大百分比。水性部分也显着显示（和 0.01) 比乙酸乙酯和氯仿馏分更高的温度降低。粗提物的自由基清除活性在最大剂量下也显着提高，而水相部分表现出显着最高的抗氧化活性。结论。总的来说，从本研究中获得的数据表明，该提取物具有显着的解热和抗氧化活性，符合传统上声称的植物用途。

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Pharmacological Evaluation of Antipyretic and Antioxidant Activities of 80% Methanol Root Extract and Derived Solvent Fraction of Echinops kebericho M. (Asteraceae) in Mice Model

Background. Toxicity and untoward effects are very ostensible in most standard drugs including antipyretic agents. Searching for conceivable antipyretic drugs with minimal toxicities and side effects from traditional plants is a growing concern to date. Echinops kebericho M. (Asteraceae) is one of the most prominent traditional medicinal plants, which is frequently testified for its traditionally claimed uses of treating fever and different infectious and noninfectious disorders by traditional healers in Ethiopian folk medicine. However, this plant has not been scientifically assessed for its traditionally claimed uses. This study therefore is aimed at investigating the antipyretic and antioxidant activities of 80% methanol root extract and the derived solvent fraction of Echinops kebericho M. in mouse models. Methods. Successive solvent maceration with increased polarity was used as the method of extractions, and chloroform, ethyl acetate, methanol, and water were used as solvents. After extraction, the crude extract and its derived solvent fractions were assessed for their antipyretic activities using yeast-induced pyrexia while, the antioxidant activities were measured in vitro using the diphenyl-2-picrylhydrazyl (DPPH) assay method. Both the extract and solvent fractions were evaluated at the doses of 100, 200, and 400 mg/kg for its antipyretic activities, and the antioxidant activity was evaluated at the doses of 50, 100, 200, 400, 600, 800, and 1000 mg/kg. The positive control group was treated with standard drug (ASA 100 mg/kg), while normal saline-receiving groups were assigned as negative control. Result. E. kebericho crude extract along with its derived solvent fractions showed statistically significant (, 0.01, and 0.001) temperature reduction activities. The maximum percentage of temperature reduction was observed by the highest dose (400 mg/kg) of the crude extract. The aqueous fraction also showed significantly ( and 0.01) higher temperature reduction than those of ethyl acetate and chloroform fractions. The free radical scavenging activities of the crude extract were also significantly high at the maximum dose, and the aqueous fraction showed the significantly highest antioxidant activity. Conclusion. In general, the data obtained from the present study clarified that the extract possessed significant antipyretic and antioxidant activities, upholding the traditionally claimed use of the plant.

更新日期：2021-03-16

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