通过聚 (PEGMA-co-GMA) 移植对后囊膜混浊抑制进行简便的多功能 IOL 表面改性,RSC Advances

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通过聚 (PEGMA-co-GMA) 移植对后囊膜混浊抑制进行简便的多功能 IOL 表面改性
RSC Advances ( IF 3.9 ) Pub Date : 2021-3-8 , DOI: 10.1039/d1ra00201e
Jiayi Xia ₁ , Duoduo Lu ₁ , Yuemei Han ₁ , Jiahao Wang ₁ , Yueze Hong ₁ , Peiyi Zhao ₁ , Qiuna Fang ₁ , Quankui Lin ₁

Affiliation

后囊膜混浊（PCO）是白内障手术中人工晶状体（IOL）植入的重要并发症，其中晶状体上皮细胞（LECs）在植入的人工晶状体表面的粘附和增殖起重要作用。IOL的表面改性以防止LEC粘附和增殖是降低PCO发生率的实用方法。在这项研究中，合成了聚（乙二醇）甲基丙烯酸酯（PEGMA）和甲基丙烯酸缩水甘油酯（GMA）的多功能二元共聚物（聚（PEGMA- co -GMA），PPG），并利用偶联化学接枝到氨基化的 IOL 表面。环氧基和氨基反应。多柔比星 (DOX) 随后通过以下方式固定在表面涂层上环氧基和氨基的反应也是如此。利用共聚物涂层中PEG链段的亲水性和DOX的抗增殖作用，合成的共聚物PPG很容易建立多功能表面涂层。这种抗增殖药物固定化亲水涂层修饰可以有效地降低细胞粘附和增殖，因此推测在PCO抑制方面具有很大的潜力。PPG的合成通过质子核磁共振光谱( ¹ H-NMR)和傅里叶变换红外光谱(FTIR)证实。通过 X 射线光电子能谱 (XPS) 证明了表面涂层的固定化。体外的还研究了药物释放曲线和细胞行为，以验证多功能涂层对细胞粘附和抗增殖的抑制作用。最后，对兔眼进行体内眼部植入，评估涂层改性人工晶状体对术后PCO的抑制作用。由此可见，这种多功能涂层改性可以有效抑制LECs的粘附和增殖，显着降低PCO的发生率。所有这些结果表明，这种 PPG 共聚物改性提供了一种简便而有效的方法来抑制 IOL 植入后 PCO 的形成。

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Facile multifunctional IOL surface modification via poly(PEGMA-co-GMA) grafting for posterior capsular opacification inhibition

Posterior capsule opacification (PCO) is a significant complication of intraocular lens (IOL) implantation in cataract surgery, in which the adhesion and proliferation of lens epithelial cells (LECs) on the implanted IOL surface play an important role. The surface modification of IOL to prevent LEC adhesion and proliferation is a practical way to reduce the incidence of PCO. In this study, a multifunctional binary copolymer of poly(ethylene glycol) methacrylate (PEGMA) and glycidyl methacrylate (GMA) was synthesized (poly(PEGMA-co-GMA), PPG) and chemically grafted onto the aminolyzed IOL surface, utilizing the coupling reaction of epoxy and amino groups. Doxorubicin (DOX) was subsequently immobilized on the surface coating via the reaction of epoxy and amino groups as well. Taking advantages of the hydrophilicity of the PEG segments in the copolymer coating and the anti-proliferative effects of the DOX, a multifunctional surface coating was easily established by the synthesized copolymer PPG. Such anti-proliferative drug immobilized hydrophilic coating modification may effectively reduce the cell adhesion and proliferation and thus it is hypothesized to have great potential in PCO inhibition. The synthesis of PPG was confirmed by proton nuclear magnetic resonance spectroscopy (¹H-NMR) and Fourier transform infrared spectroscopy (FTIR). The surface coating immobilization was demonstrated by X-ray photoelectron spectroscopy (XPS). The in vitro drug release profiles and the cell behaviors were also investigated to validate the multifunctional coating inhibition effect on cellular adhesion and antiproliferation. Finally, the in vivo ocular implantation was carried out on rabbit eyes to evaluate the effect of the coating modified IOL on the inhibition of postoperative PCO. It followed that such multifunctional coating modification can effectively inhibit the adhesion and proliferation of LECs and significantly reduce the incidence of PCO. All these results reveal that such PPG copolymer modification provides a facile yet effective way to inhibit PCO formation after IOL implantation.

更新日期：2021-03-08

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