两种刺五加叶的综合植物化学分析和镇静催眠活性,Food & Function

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两种刺五加叶的综合植物化学分析和镇静催眠活性
Food & Function ( IF 5.1 ) Pub Date : 2021-2-5 , DOI: 10.1039/d0fo02814b
Yunhe Liu ₁ , Zhongyao Wang ₁ , Caixia Wang ₁ , Hanrui Si ₁ , Hui Yu ₁ , Le Li ₁ , Shuzheng Fu ₁ , Luying Tan ₁ , Pingya Li ₁ , Jinping Liu ₁ , Yan Zhao ₂

Affiliation

刺五加叶（S _C L）和刺五加叶（S _FL）通常制成功能性茶，具有类似的药理活性。为了揭示其化学成分并评估其镇静催眠作用，基于超高效液相色谱结合四极杆飞行时间串联质谱（UPLC-Q / TOF-MS）的综合代谢物分析分析和首次进行了具有蒸发光散射检测（HPLC-ELSD）的高效液相色谱法以及小鼠的生物测定研究。首先，鉴定或简要鉴定了总共75种化合物（包括69种共有组分）。结果表明，两个物种的叶片都富含植物化学物质，并且具有相似的结构类型。其次，图20个7的化学标记物选自S鉴定_ÇL和S _F L分别。五个齐墩果烷型三萜皂苷（ciwujianoside C1，C3，D2，E和Saniculoside N）和两个羽扇豆型三萜皂苷（1-deoxychiisanoside和24-hydroxychiisanoside）可用于快速鉴定S _C L和S _F L.第三，S _C L中芦丁，二十碳三烯苷D，环戊糖苷B，-C3，-E和熊果酸的含量（分别为0.308％，0.024％，0.042％，0.131％，0.038％和0.255％）高于S _F L（分别为0.067％，0.005％，0.012％，0.015％，0.002％和0.087％）。第四，体内生物测定法验证了S _C L和S _FL可以剂量依赖的方式抑制自主活动，缩短睡眠潜伏期并延长睡眠时间。S _C L在一定程度上显示出更高且更稳定的效果。氟马西尼（FLU）可以抑制催眠作用。这两片叶子不仅具有对氯苯氧乙酸（pCPA），但还显示了与5-羟色氨酸（5-HTP）的协同催眠作用。有益的生物活性可以由5-羟基色胺（5-HT）和γ-氨基丁酸（GABA）介导。最后，网络药理分析表明，未分化和分化的化合物是两片叶子相似和不同活性的物质基础。一些典型的化学标记物（如Saniculoside N，hederacoside D，ciwujianoside C3，-E和ursolic acid，24-hydroxychiisanoside和1-deoxyisochiisanoside）是潜在的活性化合物，将来可以用作质量标记。本研究为这两种刺五加叶的进一步开发和利用提供了基础。

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Comprehensive phytochemical analysis and sedative-hypnotic activity of two Acanthopanax species leaves

Acanthopanax senticosus leaves (S_CL) and Acanthopanax sessiliflorus leaves (S_FL), which are usually made into functional teas, possess similar pharmacological activities. With the aim of revealing their chemical compositions and evaluating their sedative-hypnotic effects, comprehensive metabolite profiling analysis based on ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry (UPLC-Q/TOF-MS) and high-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) as well as bioassay studies in mice were performed for the first time. Firstly, a total of 75 compounds (including 69 shared components) were identified or briefly characterized. Results indicated that the leaves of the two species were both rich in phytochemicals and contained similar structural types. Secondly, 20 and 7 chemical markers were identified from S_CL and S_FL, respectively. Five oleanane-type triterpene saponins (ciwujianoside C1, C3, D2, E and saniculoside N) and two lupine-type triterpene saponins (1-deoxychiisanoside and 24-hydroxychiisanoside) may be used for rapid identification of S_CL and S_FL. Thirdly, the contents of rutin, hederacoside D, ciwujianoside B, -C3, -E and ursolic acid in S_CL (0.308%, 0.024%, 0.042%, 0.131%, 0.038%, and 0.255%, respectively) were higher than in S_FL (0.067%, 0.005%, 0.012%, 0.015%, 0.002%, and 0.087%, respectively). Fourthly, an in vivo bioassay verified that both S_CL and S_FL could inhibit autonomous activity, shorten sleep latency and prolong sleep duration in a dose-dependent manner. To a certain degree, S_CL showed a higher and more stable effect. The hypnotic effect could be inhibited by flumazenil (FLU). The two leaves not only had an obvious antagonism action of p-chlorophenoxyacetic acid (pCPA) but also showed a synergistic hypnotic effect with 5-hydroxytryptophan (5-HTP). The beneficial bioactivity may be mediated by 5-hydroxytryptamine (5-HT) and γ-aminobutyric acid (GABA). Finally, network pharmacology analysis showed that the undifferentiated and differentiated compounds were the material basis for the similar and the different activities of two leaves. Some typical chemical markers (such as saniculoside N, hederacoside D, ciwujianoside C3, -E and ursolic acid, 24-hydroxychiisanoside and 1-deoxyisochiisanoside) were the potential active compounds and could be used as quality markers in the future. The present study furnished a basis for the further development and utilization of the leaves of these two Acanthopanax species.

更新日期：2021-02-19

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