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Simple Colorimetric and Fluorescence Chemosensing Probe for Selective Detection of Sn2+ Ions in an Aqueous Solution: Evaluation of the Novel Sensing Mechanism and Its Bioimaging Applications
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-12-07 , DOI: 10.1021/acs.analchem.0c03196
Palanisamy Ravichandiran 1 , Vignesh Krishnamoorthi Kaliannagounder 2, 3 , Antony Paulraj Bella 4 , Anna Boguszewska-Czubara 5 , Maciej Masłyk 6 , Cheol Sang Kim 2, 7 , Chan Hee Park 2, 7 , Princy Merlin Johnson 4 , Byung-Hyun Park 8 , Myung-Kwan Han 9 , Ae Rhan Kim 1, 10 , Dong Jin Yoo 1, 10
Affiliation  

An easily accessible colorimetric and fluorescence probe 4-((3-chloro-1,4-dioxo-1,4-dihydronaphthalen-2-yl)amino)benzenesulfonamide (4CBS) was successfully developed for the selective and sensitive detection of Sn2+ in an aqueous solution. The sensing mechanism involves reduction of −C═O into −C–OH groups in 4CBS upon the addition of Sn2+, which initiates the fluorescence turn-on mode. A better linear relationship was achieved between fluorescence intensity and Sn2+ concentration in the range of 0–62.5 μM, with a detection limit (LOD) of 0.115 μM. The binding mechanism of 4CBS for Sn2+ was confirmed by Fourier transform infrared analysis, NMR titrations, and mass (electrospray ionization) spectral analysis. Likewise, the proposed sensing mechanism was supported by quantum chemical calculations. Moreover, bioimaging studies demonstrated that the chemosensing probe 4CBS is an effective fluorescent marker for the detection of Sn2+ in living cells and zebrafish. Significantly, 4CBS was able to discriminate between Sn2+ in human cancer cells and Sn2+ in normal live cells.

中文翻译:

用于溶液中Sn 2+离子选择性检测的简单比色和荧光化学传感探针:新型传感机制及其生物成像应用的评估

成功开发出一种易于使用的比色和荧光探针4-((3-氯-1,4-二氧-1,4-二氢萘-2-基)氨基)苯磺酰胺(4CBS)用于选择性和灵敏地检测Sn 2+在水溶液中。传感机制涉及在添加Sn 2+时,将4 CBS中的-C═O还原为-C-OH基团,从而启动荧光开启模式。荧光强度和Sn 2+浓度在0-62.5μM范围内具有更好的线性关系,检出限(LOD)为0.115μM。4CBS与Sn 2+的结合机理通过傅立叶变换红外分析,NMR滴定和质谱(电喷雾电离)光谱分析得到证实。同样,拟议的传感机制得到了量子化学计算的支持。此外,生物成像研究表明,化学传感探针4CBS是检测活细胞和斑马鱼中Sn 2+的有效荧光标记。显著,4CBS能够锡区分2+在人类癌细胞和Sn 2+在正常活细胞。
更新日期:2021-01-19
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