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Continuous Enzymatic Synthesis of 2-Ethylhexyl Oleate in a Fluidized Bed Reactor: Operating Conditions, Hydrodynamics, and Mathematical Modeling
Industrial & Engineering Chemistry Research ( IF 3.8 ) Pub Date : 2020-10-21 , DOI: 10.1021/acs.iecr.0c03504 Mateus V. C. da Silva 1 , Amanda B. S. Rangel 1 , Leandro G. Aguiar 1 , Heizir F. de Castro 1 , Larissa de Freitas 1
Industrial & Engineering Chemistry Research ( IF 3.8 ) Pub Date : 2020-10-21 , DOI: 10.1021/acs.iecr.0c03504 Mateus V. C. da Silva 1 , Amanda B. S. Rangel 1 , Leandro G. Aguiar 1 , Heizir F. de Castro 1 , Larissa de Freitas 1
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The continuous synthesis of 2-ethylhexyl oleate catalyzed by Candida antarctica lipase immobilized on magnetic poly(styrene-co-divinylbenzene) particles was performed in a solvent-free fluidized bed reactor (FBR). The effects of space time (6, 12, and 18 h) and bed porosity (0.866, 0.892, and 0.916) were investigated to determine the best operating conditions. Experimental results showed that high ester productivity (0.73 mmol g–1 h–1) and a satisfactory esterification yield (48.24%) were achieved at a space time of 12 h and a bed porosity of 0.892. It was also observed that the higher the bed porosity, the higher the mass transfer coefficient and, consequently, the reactor productivity. This finding can be explained by the high interstitial velocity of the fluidized bed bioreactor, which increases the mixing degree and improves mass transfer. A mathematical model accounting for internal and external particle transfer effects was proposed based on ping-pong bi–bi enzyme kinetics. The FBR was described in a simplified manner, considering an agitated tank reactor, a strategy that provided good correlation (R2 = 0.991) between experimental and model results. Immobilized lipase showed high operational stability (half-life of 1716 h) qualifying as a promising biocatalyst for industrial-scale applications.
中文翻译：
在流化床反应器中连续酶法合成油酸2-乙基己酯：操作条件，流体力学和数学模型
在无溶剂流化床反应器（FBR）中，固定化在磁性聚（苯乙烯-共-二乙烯基苯）颗粒上的南极假丝酵母脂肪酶催化连续合成油酸2-乙基己酯。研究了时空（6、12和18 h）和床孔隙率（0.866、0.892和0.916）的影响，以确定最佳操作条件。实验结果表明，高酯产率（0.73 mmol g –1 h –1在12 h的时空和0.892的床孔隙率下获得了令人满意的酯化收率（48.24％）。还观察到床孔隙率越高，传质系数越高，因此反应器的生产率越高。该发现可以通过流化床生物反应器的高间隙速度来解释，该间隙速度增加了混合度并改善了质量传递。提出了一种基于乒乓bi-bi酶动力学的内部和外部颗粒转移效应的数学模型。考虑到搅拌釜反应器，以简化的方式描述了FBR，该策略可提供良好的相关性（R 2= 0.991）。固定化脂肪酶显示出高的操作稳定性（半衰期为1716小时），可作为工业规模应用中有希望的生物催化剂。
更新日期：2020-11-04
中文翻译：
在流化床反应器中连续酶法合成油酸2-乙基己酯：操作条件，流体力学和数学模型
在无溶剂流化床反应器（FBR）中，固定化在磁性聚（苯乙烯-共-二乙烯基苯）颗粒上的南极假丝酵母脂肪酶催化连续合成油酸2-乙基己酯。研究了时空（6、12和18 h）和床孔隙率（0.866、0.892和0.916）的影响，以确定最佳操作条件。实验结果表明，高酯产率（0.73 mmol g –1 h –1在12 h的时空和0.892的床孔隙率下获得了令人满意的酯化收率（48.24％）。还观察到床孔隙率越高，传质系数越高，因此反应器的生产率越高。该发现可以通过流化床生物反应器的高间隙速度来解释，该间隙速度增加了混合度并改善了质量传递。提出了一种基于乒乓bi-bi酶动力学的内部和外部颗粒转移效应的数学模型。考虑到搅拌釜反应器，以简化的方式描述了FBR，该策略可提供良好的相关性（R 2= 0.991）。固定化脂肪酶显示出高的操作稳定性（半衰期为1716小时），可作为工业规模应用中有希望的生物催化剂。
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