A novel near-infrared BODIPY-based fluorescent probe MPBOD was designed and synthesized. MPBOD can be used to minitor carboxylesterase1 (CES1) activities in living cells. MPBOD has high selectivity to CES1 and its detection mechanism is based on the spontaneous hydrolysis of carboxylate bonds catalyzed by (CES1). Compared with the only few reported CES1 probes, MPBOD showed high sensitivity, low background interference, low cytotoxicity and good biocompatibility. Additionally, MPBOD was successfully applied to detect carbamates pesticide (carbaryl) as well as monitor the real activities of CES1 in lysosome of HepG2 cells. This type of probe can be a promising tool to visualize many pathological processes because CES1 is involved in the biotransformation of drugs and poisons with ester groups.

设计并合成了一种新型的基于BODIPY的近红外荧光探针MPBOD。MPBOD可用于减少活细胞中的羧酸酯酶1（CES1）活性。MPBOD对CES1具有高选择性，其检测机理是基于（CES1）催化的羧酸酯键的自发水解。与报道的只有少数CES1探针相比，MPBOD具有高灵敏度，低背景干扰，低细胞毒性和良好的生物相容性。此外，MPBOD已成功地应用于检测氨基甲酸酯类农药（西维因）以及监测HepG2细胞溶酶体中CES1的真实活性。这种类型的探针可能是可视化许多病理过程的有前途的工具，因为CES1参与具有酯基的药物和毒物的生物转化。