Activating Acid‐Sensing Ion Channels with Photoacid Generators,ChemPhotoChem

当前位置： X-MOL 学术 › ChemPhotoChem › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Activating Acid‐Sensing Ion Channels with Photoacid Generators
ChemPhotoChem ( IF 3.0 ) Pub Date : 2020-08-20 , DOI: 10.1002/cptc.202000154
Xinglei Liu ₁ , Karthik Sambath ₁ , Lauren Hutnik ₁ , Jianyang Du ₂ , Kevin D Belfield ₁ , Yuanwei Zhang ₁

Affiliation

Acid‐sensing ion channels (ASICs), present in both central and peripheral neurons, respond to changes in extracellular protons. They play important roles in many symptoms and diseases, such as pain, ischemic stroke and neurodegenerative diseases. Herein, we report a novel approach to activate ASICs with the precision of light using organic photoacid generators (PAGs), which are molecules that release H⁺ upon light illumination, and have been recently used in biomedical studies. The PAGs showed low toxicity in dark conditions. Under LED light illumination, ASICs activation and consequent calcium ion influx was monitored and analysed by fluorescence microscopy, and showed a strong light‐dependent response. This approach allows the activation of ASICs with the precision of light, and may be valuable to help better elucidate the molecular mechanism of ASICs and unveil their roles in physiology, pathophysiology, and behaviour.

中文翻译：

用光酸发生器激活酸敏感离子通道

酸敏感离子通道（ASIC）存在于中枢和外周神经元中，对细胞外质子的变化做出反应。它们在许多症状和疾病中发挥着重要作用，例如疼痛、缺血性中风和神经退行性疾病。在此，我们报告了一种利用有机光酸发生器 (PAG) 精确激活 ASIC 的新方法，有机光酸发生器 (PAG) 是在光照射下释放 H ⁺的分子，最近已用于生物医学研究。 PAG 在黑暗条件下表现出低毒性。在 LED 光照射下，通过荧光显微镜监测和分析 ASIC 的激活和随之而来的钙离子流入，并显示出强烈的光依赖性响应。这种方法可以通过光精确激活 ASIC，并且可能有助于更好地阐明 ASIC 的分子机制并揭示它们在生理学、病理生理学和行为中的作用。

更新日期：2020-08-20

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文