The pre‐mRNA splicing factor RDM16 regulates root stem cell maintenance in Arabidopsis,Journal of Integrative Plant Biology

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The pre‐mRNA splicing factor RDM16 regulates root stem cell maintenance in Arabidopsis
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-08-13 , DOI: 10.1111/jipb.13006
Bingsheng Lv ₁ , Kongqin Hu ₁ , Te Tian ₁ , Kaijing Wei ₁ , Feng Zhang ₁ , Yuebin Jia _{1,

2} , Huiyu Tian ₁ , Zhaojun Ding ₁

Affiliation

Pre‐mRNA (messenger RNA) splicing participates in the regulation of numerous biological processes in plants. For example, alternative splicing shapes transcriptomic responses to abiotic and biotic stress, and controls developmental programs. However, no study has revealed a role for splicing in maintaining the root stem cell niche. Here, a screen for defects in root growth in Arabidopsis thaliana identified an ethyl methane sulfonate mutant defective in pre‐mRNA splicing (rdm16‐4). The rdm16‐4 mutant displays a short‐root phenotype resulting from fewer cells in the root apical meristem. The PLETHORA1 (PLT1) and PLT2 transcription factor genes are important for root development and were alternatively spliced in rdm16‐4 mutants, resulting in a disordered root stem cell niche and retarded root growth. The root cap of rdm16‐4 contained reduced levels of cytokinins, which promote differentiation in the developing root. This reduction was associated with the alternative splicing of genes encoding cytokinin signaling factors, such as ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN5 and ARABIDOPSIS RESPONSE REGULATORS (ARR1, ARR2, and ARR11). Furthermore, expression of the full‐length coding sequence of ARR1 or exogenous cytokinin application partially rescued the short‐root phenotype of rdm16‐4. This reveals that the RDM16‐mediated alternative splicing of cytokinin signaling components contributes to root growth.

中文翻译：

前mRNA剪接因子RDM16调节拟南芥根干细胞的维持

Pre-mRNA（信使RNA）剪接参与了植物中许多生物过程的调节。例如，选择性剪接形成对非生物和生物胁迫的转录组反应，并控制发育程序。然而，没有研究揭示剪接在维持根干细胞生态位方面的作用。在这里，对拟南芥根生长缺陷的筛选鉴定了一种前体 mRNA 剪接缺陷的甲磺酸乙酯突变体 ( rdm16-4 )。所述rdm16-4突变体显示的短根表型从在根顶端分生组织的细胞较少产生。所述PLETHORA1（PLT1）和PLT2转录因子基因对根发育很重要，并且在rdm16-4突变体中选择性剪接，导致根干细胞生态位紊乱和根生长受阻。的根冠rdm16-4包含减少细胞分裂素的水平，这促进在显影根分化。这种减少与编码细胞分裂素信号因子的基因的选择性剪接有关，例如ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN5和ARABIDOPSIS RESPONSE REGULATORS（ARR1、ARR2和ARR11）。此外，ARR1全长编码序列的表达或外源性细胞分裂素应用部分挽救了rdm16-4的短根表型。这表明 RDM16 介导的细胞分裂素信号组分的选择性剪接有助于根生长。

更新日期：2020-08-13

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