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Extension of cell membrane boosting squalene production in the engineered Escherichia coli.
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2020-07-21 , DOI: 10.1002/bit.27511 Yunhe Meng 1 , Xixi Shao 1 , Yan Wang 1 , Yumei Li 1 , Xiaojian Zheng 1 , Gongyuan Wei 1 , Seon-Won Kim 2 , Chonglong Wang 1
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2020-07-21 , DOI: 10.1002/bit.27511 Yunhe Meng 1 , Xixi Shao 1 , Yan Wang 1 , Yumei Li 1 , Xiaojian Zheng 1 , Gongyuan Wei 1 , Seon-Won Kim 2 , Chonglong Wang 1
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Squalene is a lipophilic and non‐volatile triterpene with many industrial applications for food, pharmaceuticals, and cosmetics. Metabolic engineering focused on optimization of the production pathway suffer from little success in improving titers because of a limited space of the cell membrane accommodating the lipophilic product. Extension of cell membrane would be a promising approach to overcome the storage limitation for successful production of squalene. In this study, Escherichia coli was engineered for squalene production by overexpression of some membrane proteins. The highest production of 612 mg/L was observed in the engineered E. coli with overexpression of Tsr, a serine chemoreceptor protein, which induced invagination of inner membrane to form multilayered structure. It was also observed an increase in unsaturated fatty acid in membrane lipids composition, suggesting cellular response to maintain membrane fluidity against squalene accumulation in the engineered strain. This study potentiates the capability of E. coli for squalene production and provides an effective strategy for the enhanced production of such compounds.
中文翻译：
细胞膜的延伸促进了工程大肠杆菌中角鲨烯的产生。
角鲨烯是一种亲脂性和非挥发性三萜，在食品、药品和化妆品等工业领域有许多应用。由于容纳亲脂性产物的细胞膜空间有限，专注于优化生产途径的代谢工程在提高滴度方面几乎没有成功。细胞膜的延伸将是克服成功生产角鲨烯的储存限制的有前途的方法。在这项研究中，大肠杆菌被设计用于通过过度表达一些膜蛋白来生产角鲨烯。在工程化大肠杆菌中观察到最高产量为 612 mg/L 。大肠杆菌与丝氨酸化学感受器蛋白 Tsr 的过表达，其诱导内膜内陷形成多层结构。还观察到膜脂质组成中不饱和脂肪酸的增加，表明细胞反应维持膜流动性对抗工程菌株中角鲨烯的积累。该研究增强了大肠杆菌生产角鲨烯的能力，并为增强此类化合物的生产提供了有效的策略。
更新日期：2020-07-21
中文翻译：
细胞膜的延伸促进了工程大肠杆菌中角鲨烯的产生。
角鲨烯是一种亲脂性和非挥发性三萜，在食品、药品和化妆品等工业领域有许多应用。由于容纳亲脂性产物的细胞膜空间有限，专注于优化生产途径的代谢工程在提高滴度方面几乎没有成功。细胞膜的延伸将是克服成功生产角鲨烯的储存限制的有前途的方法。在这项研究中，大肠杆菌被设计用于通过过度表达一些膜蛋白来生产角鲨烯。在工程化大肠杆菌中观察到最高产量为 612 mg/L 。大肠杆菌与丝氨酸化学感受器蛋白 Tsr 的过表达，其诱导内膜内陷形成多层结构。还观察到膜脂质组成中不饱和脂肪酸的增加，表明细胞反应维持膜流动性对抗工程菌株中角鲨烯的积累。该研究增强了大肠杆菌生产角鲨烯的能力，并为增强此类化合物的生产提供了有效的策略。
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