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FORGETTER2 protein phosphatase and phospholipase D modulate heat stress memory in Arabidopsis.
The Plant Journal ( IF 6.2 ) Pub Date : 2020-07-12 , DOI: 10.1111/tpj.14927 Reynel Urrea Castellanos 1 , Thomas Friedrich 1 , Nevena Petrovic 1 , Simone Altmann 1 , Krzysztof Brzezinka 1 , Michal Gorka 2 , Alexander Graf 2 , Isabel Bäurle 1
The Plant Journal ( IF 6.2 ) Pub Date : 2020-07-12 , DOI: 10.1111/tpj.14927 Reynel Urrea Castellanos 1 , Thomas Friedrich 1 , Nevena Petrovic 1 , Simone Altmann 1 , Krzysztof Brzezinka 1 , Michal Gorka 2 , Alexander Graf 2 , Isabel Bäurle 1
Affiliation
Plants can mitigate environmental stress conditions through acclimation. In the case of fluctuating stress conditions such as high temperatures, maintaining a stress memory enables a more efficient response upon recurring stress. In a genetic screen for Arabidopsis thaliana mutants impaired in the memory of heat stress (HS) we have isolated the FORGETTER2 (FGT2) gene, which encodes a type 2C protein phosphatase (PP2C) of the D‐clade. Fgt2 mutants acquire thermotolerance normally; however, they are defective in the memory of HS. FGT2 interacts with phospholipase D α2 (PLDα2), which is involved in the metabolism of membrane phospholipids and is also required for HS memory. In summary, we have uncovered a previously unknown component of HS memory and identified the FGT2 protein phosphatase and PLDα2 as crucial players, suggesting that phosphatidic acid‐dependent signaling or membrane composition dynamics underlie HS memory.
中文翻译:
FORGETTER2蛋白磷酸酶和磷脂酶D调节拟南芥中的热应激记忆。
植物可以通过适应环境减轻环境压力。在波动的应力条件(例如高温)的情况下,保持应力记忆可实现对重复应力的更有效响应。在热应激(HS)记忆受损的拟南芥突变体的遗传筛选中,我们分离了FORGETTER2(FGT2)基因,该基因编码D进化枝的2C型蛋白磷酸酶(PP2C)。Fgt2突变体通常获得耐热性;但是,它们在HS的存储器中有缺陷。FGT2与磷脂酶Dα2(PLDα2)相互作用,后者与膜磷脂的代谢有关,也是HS记忆所必需的。总而言之,我们发现了HS记忆的一个先前未知的组成部分,并将FGT2蛋白磷酸酶和PLDα2鉴定为至关重要的参与者,这表明HS记忆是磷脂酸依赖性信号或膜组成动力学的基础。
更新日期:2020-07-12
中文翻译:
FORGETTER2蛋白磷酸酶和磷脂酶D调节拟南芥中的热应激记忆。
植物可以通过适应环境减轻环境压力。在波动的应力条件(例如高温)的情况下,保持应力记忆可实现对重复应力的更有效响应。在热应激(HS)记忆受损的拟南芥突变体的遗传筛选中,我们分离了FORGETTER2(FGT2)基因,该基因编码D进化枝的2C型蛋白磷酸酶(PP2C)。Fgt2突变体通常获得耐热性;但是,它们在HS的存储器中有缺陷。FGT2与磷脂酶Dα2(PLDα2)相互作用,后者与膜磷脂的代谢有关,也是HS记忆所必需的。总而言之,我们发现了HS记忆的一个先前未知的组成部分,并将FGT2蛋白磷酸酶和PLDα2鉴定为至关重要的参与者,这表明HS记忆是磷脂酸依赖性信号或膜组成动力学的基础。