In this paper, a high-performance enzyme cascade bio-platform has been developed for biosensing by combining MOFs-based nanozyme and natural enzymes. Firstly, a novel porous mixed bi-metal oxide (MnCo₂O₄) derived from MOF with rod-like nanostructures was synthesized. Based on this, the nanozyme of bovine serum albumin-Pt nanoparticles@mesoporous MnCo₂O₄ (BSA-PtNP@MnCo₂O₄) was successfully synthesized and used to construct enzyme cascade bio-platform. The nanozyme had unique physicochemical surface properties and hierarchical structure. Due to the synergistic effect of protein, bimetal oxide and PtNP, the nanozyme presented excellent dual enzyme activity. On the one hand, BSA-PtNP@MnCo₂O₄ can be used as nanozyme with oxidase activity to achieve superior detection of glutathione with detection limit of 0.42 μM. On the other hand, BSA- PtNP@MnCo₂O₄ can also be used both as the nanozyme with great peroxidase activity and as a scaffold for immobilization of glucose oxidase (GOx), guiding an organized high-efficiency enzyme cascade bio-platform. The platform combined advantages of nanozyme and natural enzyme, and provided excellent glucose detection with the detection limit of 8.1 μM. The tandem catalytic system not only broadened the application of nanozyme in natural enzyme catalysis, but also provided a simple, efficient and organized enzyme cascade bio-platform for biosensing and other applications.

通过结合基于MOFs的纳米酶和天然酶，已开发出一种用于生物传感的高性能酶级联生物平台。首先，合成了一种新型的由MOF衍生的具有杆状纳米结构的多孔混合双金属氧化物（MnCo ₂ O ₄）。基于此，牛血清白蛋白-Pt纳米粒子@中孔MnCo ₂ O ₄（BSA-PtNP @ MnCo ₂ O _4）的纳米酶）已成功合成并用于构建酶级联生物平台。纳米酶具有独特的理化表面性质和层次结构。由于蛋白质，双金属氧化物和PtNP的协同作用，纳米酶具有出色的双重酶活性。一方面，BSA-PtNP @ MnCo ₂ O ₄可以用作具有氧化酶活性的纳米酶，实现谷胱甘肽的优异检测，检测限为0.42μM。另一方面，BSA-PtNP @ MnCo ₂ O ₄既可用作具有过氧化物酶活性的纳米酶，又可用作固定葡萄糖氧化酶（GOx）的支架，可指导有组织的高效酶级联生物平台。该平台结合了纳米酶和天然酶的优势，提供了出色的葡萄糖检测能力，检测限为8.1μM。串联催化系统不仅拓宽了纳米酶在天然酶催化中的应用范围，而且为生物传感和其他应用提供了一种简单，高效，有组织的酶级联生物平台。