Pongamol a major bioactive compound present in karanja (Pongamia pinnata) seed oil, is a polyphenolic chalcone belonging to the flavonoid family. It is attributed to having several medicinal and therapeutic properties. This study reports a feasible technique to efficiently extract pongamol from karanja seed oil by treating with boron trifluoride etherate (BF₃·OEt₂) and its anti-inflammatory activity. This method affords 64 % recovery of pongamol with >95 % purity (HPLC) and it is characterized by FTIR and NMR spectroscopy. The IC₅₀ value for soy lipoxygenase-1 (LOX-1) inhibitory activity was found to be 72.2 μM. Interaction of pongamol with soy LOX-1 by Fluorescence revealed that a quenching maximum of 50.25 % with the binding constant of 0.75×10⁵M^-1 . In silico studies revealed that pongamol could bind with soy LOX-1 with least binding energy of −5.79 kcal/mol by forming two hydrogen bonds. Pongamol exhibited antioxidant activity with an IC₅₀ value of 12.2 μg/mL. In this investigation, semi-synthesis of dihydropongamol (DHP), a new molecule and its acute anti-inflammatory property is reported. Edema was induced in paw and ear of Wistar rats using Carrageenan and xylene. Pongamol of 50 ppm body weight reduced the edema by 55 % in the paw and 74 % in the ear within 3 h of its administration. The rat groups fed with dihydropongamol also exhibited similar anti-inflammatory activities. Primarily, this investigation reports a practical approach to pongamol isolation and also for the first time its feasibility as a potent anti-inflammatory agent.

Pongamol是karanja（Pongamia pinnata）种子油中存在的主要生物活性化合物，是一种属于类黄酮家族的多酚查尔酮。它归因于具有几种医学和治疗特性。这项研究报告了一种可行的技术，可以通过用三氟化硼醚化物（BF ₃ ·OEt ₂）处理从魔芋种子油中有效提取五加酚，并具有抗炎活性。此方法可回收64％的pongamol，纯度> 95％（HPLC），并通过FTIR和NMR光谱进行表征。IC ₅₀大豆脂氧合酶-1（LOX-1）抑制活性的值为72.2μM。pongamol与大豆LOX-1的荧光相互作用表明，猝灭最大值为50.25％，结合常数为0.75×10 ⁵ M ^-1。在计算机研究中，pongamol可以通过形成两个氢键与大豆LOX-1结合，结合能最小为-5.79 kcal / mol。庞加莫尔具有IC _50的抗氧化活性值为12.2μg/ mL。在这项研究中，报道了一种新分子半合成的双氢pongamol（DHP）及其急性抗炎特性。使用角叉菜胶和二甲苯在Wistar大鼠的爪和耳朵中引起水肿。体重为50 ppm的Pongamol在给药后3小时内可将爪中的水肿减少55％，将耳朵中的水肿减少74％。用二氢pongamol喂养的大鼠组也表现出相似的抗炎活性。首先，该研究报告了一种用于分离pongamol的实用方法，并且首次报道了其作为有效抗炎剂的可行性。