The synthesized Fe-MIL-88NH₂ was used as a nanozyme and fluorescence probe to successfully construct a selective fluorescent sensing platform for one step determination of catechol. Fe-MIL-88NH₂ exhibited excellent peroxidase mimic property as well as fluorescence property. With the addition of H₂O₂ and catechol, Fe-MIL-88NH₂ would catalyze the H₂O₂ to form hydroxyl radicals (OH). Then, the oxidation of catechol would be triggered by the formed OH, which resulted in the generation of 1, 2-benzoquinone. Afterwards, Michael reaction between the oxidation products of catechol (1, 2-benzoquinone) and Fe-MIL-88NH₂ ligands was triggered and then quenched the fluorescence of Fe-MIL-88NH₂. The principle of the selective determination of catechol was due to the assistant effect of Michael reaction. Based on the above sensing mechanism, a fluorescent sensing platform for the determination of catechol was established. The limit of detection was 0.0913 μmol L⁻¹ (3S_{0, n=8}/K) within a fast response of 10 min. This simple and selective strategy, based on the Michael reaction and the superiority of Fe-MIL-88NH₂ nanozyme, provided a concise sensing platform for catechol determination, which broadened the scope of application of nanozyme and showed a great potential application for on-site testing in food and biology analysis.

合成的Fe-MIL-88NH ₂用作纳米酶和荧光探针成功地构建了选择性荧光传感平台，用于一步测定邻苯二酚。Fe-MIL-88NH ₂表现出优异的过氧化物酶模拟性质以及荧光性质。通过添加H ₂ O ₂和邻苯二酚，Fe-MIL-88NH ₂将催化H ₂ O ₂形成羟基自由基（OH）。然后，形成的OH会引发邻苯二酚的氧化，从而导致生成1，2-苯并醌。之后，邻苯二酚（1，2-苯醌）的氧化产物与Fe-MIL-88NH ₂之间的迈克尔反应触发配体，然后淬灭Fe-MIL-88NH ₂的荧光。选择性测定儿茶酚的原理是由于迈克尔反应的辅助作用。基于上述检测机理，建立了测定儿茶酚的荧光检测平台。在10分钟的快速响应内，检出限为0.0913μmolL ^-1（3S _{0，n = 8} / K）。这种简单，选择性的策略基于迈克尔反应和Fe-MIL-88NH ₂纳米酶的优越性，为儿茶酚的测定提供了一个简洁的传感平台，拓宽了纳米酶的应用范围，显示了在现场的巨大应用潜力。食品和生物学分析中的测试。