ECM-based microchannel for culturing in vitro vascular tissues with simultaneous perfusion and stretch.,Lab on a Chip

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ECM-based microchannel for culturing in vitro vascular tissues with simultaneous perfusion and stretch.
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-04-20 , DOI: 10.1039/d0lc00254b
Azusa Shimizu ₁ , Wei Huang Goh ₂ , Shun Itai ₁ , Michinao Hashimoto ₃ , Shigenori Miura ₄ , Hiroaki Onoe ₅

Affiliation

We present an extracellular matrix (ECM)-based stretchable microfluidic system for culturing in vitro three-dimensional (3D) vascular tissues, which mimics in vivo blood vessels. Human umbilical vein endothelial cells (HUVECs) can be cultured under perfusion and stretch simultaneously with real-time imaging by our proposed system. Our ECM (transglutaminase (TG) cross-linked gelatin)-based microchannel was fabricated by dissolving water-soluble sacrificial polyvinyl alcohol (PVA) molds printed with a 3D printer. Flows in the microchannel were analyzed under perfusion and stretch. We demonstrated simultaneous perfusion and stretch of TG gelatin-based microchannels culturing HUVECs. We suggest that our TG gelatin-based stretchable microfluidic system proves to be a useful tool for understanding the mechanisms of vascular tissue formation and mechanotransduction.

中文翻译：

基于ECM的微通道，用于同时灌注和拉伸培养体外血管组织。

我们提出了一种基于细胞外基质（ECM）的可拉伸微流体系统，用于培养体外模拟体内的三维（3D）血管组织血管。通过我们提出的系统，可以在灌注和拉伸的同时培养人脐静脉内皮细胞（HUVEC）。我们的ECM（转谷氨酰胺酶（TG）交联明胶）基微通道是通过溶解3D打印机打印的水溶性牺牲聚乙烯醇（PVA）模具制成的。在灌注和拉伸下分析微通道中的流量。我们展示了同时培养HUVEC的基于TG明胶的微通道的灌注和拉伸。我们建议，我们的基于TG明胶的可拉伸微流体系统被证明是了解血管组织形成和机械转导机制的有用工具。

更新日期：2020-04-20

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