Phylogenomics of Rhodocyclales and its distribution in wastewater treatment systems.,Scientific Reports

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Phylogenomics of Rhodocyclales and its distribution in wastewater treatment systems.
Scientific Reports ( IF 3.8 ) Pub Date : 2020-03-03 , DOI: 10.1038/s41598-020-60723-x
Zhongjie Wang ₁ , Wenqing Li ₂ , Hao Li ₁ , Wei Zheng ₁ , Feng Guo ₁

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Rhodocyclales is an abundant bacterial order in wastewater treatment systems and putatively plays key roles in multiple functions. Its phylogenomics, prevalence of denitrifying genes in sub-lineages and distribution in wastewater treatment plants (WWTPs) worldwide have not been well characterized. In the present study, we collected 78 Rhodocyclales genomes, including 17 from type strains, non-type strains and genome bins contributed by this study. Phylogenomics indicated that the order could be divided into five family-level lineages. With only a few exceptions (mostly in Rhodocyclaceae), nirS-containing genomes in this order usually contained the downstream genes of norB and nosZ. Multicopy of denitrifying genes occurred frequently and events of within-order horizontal transfer of denitrifying genes were phylogenetically deduced. The distribution of Rhodocyclaceae, Zoogloeaceae and Azonexaceae in global WWTPs were significantly governed by temperature, mixed liquor suspended solids, etc. Metagenomic survey showed that the order generally ranked at the top or second for different denitrifying genes in wastewater treatment systems. Our results provided comprehensive genomic insights into the phylogeny and features of denitrifying genes of Rhodocyclales. Its contribution to the denitrifying gene pool in WWTPs was proved.

中文翻译：

红花杜鹃的系统经济学及其在废水处理系统中的分布。

杜鹃花是废水处理系统中丰富的细菌，在多种功能中起关键作用。其系统基因组学，反硝化基因在亚系中的流行以及在全球废水处理厂（WWTP）中的分布尚未得到很好的表征。在本研究中，我们收集了78个红景天基因组，包括来自本研究贡献的类型菌株，非类型菌株和基因组仓中的17个。系统生物学研究表明，该顺序可以分为五个家族水平的谱系。除少数例外（多数在红景天科中）外，按此顺序包含nirS的基因组通常包含norB和nosZ的下游基因。反硝化基因的多拷贝频繁发生，并且反演了反硝化基因的内部水平转移的事件。全球污水处理厂中的红景天科，动植物科和阿扎克塞科的分布受温度，混合液悬浮固体等的显着控制。元基因组学研究表明，废水处理系统中不同反硝化基因的顺序通常排在第一或第二。我们的结果提供了全面的基因组见解，以了解红景天的系统发育和反硝化基因的特征。证明了其对污水处理厂反硝化基因库的贡献。我们的结果提供了全面的基因组见解，以了解红景天的系统发育和反硝化基因的特征。证明了其对污水处理厂反硝化基因库的贡献。我们的结果提供了全面的基因组见解，以了解红景天的系统发育和反硝化基因的特征。证明了其对污水处理厂反硝化基因库的贡献。

更新日期：2020-03-03

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