外源15 N标记的胸苷，脱氧腺苷，脱氧鸟苷和脱氧胞苷的掺入特征到细菌DNA中。,PLOS ONE

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外源15 N标记的胸苷，脱氧腺苷，脱氧鸟苷和脱氧胞苷的掺入特征到细菌DNA中。
PLOS ONE ( IF 2.9 ) Pub Date : 2020-02-27 , DOI: 10.1371/journal.pone.0229740
Kenji Tsuchiya _{1,

2} , Tomoharu Sano ₃ , Noriko Tomioka ₂ , Ayato Kohzu ₂ , Kazuhiro Komatsu ₂ , Ryuichiro Shinohara ₂ , Shinji Shimode ₄ , Tatsuki Toda ₁ , Akio Imai ₂

Affiliation

通常使用DNA标记的胸腺嘧啶脱氧核糖核酸从DNA合成速率估计细菌生产。一些细菌无法将胞外胸腺嘧啶脱氧核苷掺入其DNA中，这表明使用常规方法可能会忽略其生物量产生。在本研究中，为了从DNA合成的角度评估脱氧核糖核苷在评估天然细菌群落细菌生产中的适用性，将被氮稳定同位素（15N）标记的四种脱氧核糖核苷（胸苷，脱氧腺苷，脱氧鸟苷和脱氧胞苷）的掺入率提高到细菌DNA中在2015年7月和2016年1月对海洋（相模湾）和淡水（霞浦湖）生态系统进行了检查。在相模湾和霞浦湖的大多数气象站，我们发现，在四种脱氧核糖核苷中，脱氧鸟苷的掺入率最高，而脱氧鸟苷的掺入率约为胸腺嘧啶核苷的2.5倍。而脱氧腺苷和脱氧胞苷的掺入率是胸苷的0.9和0.2倍。这些结果清楚地表明，与其他脱氧核糖核苷相比，可以将外源性脱氧鸟苷掺入其DNA的细菌种类的数量相对更大，并且使用脱氧鸟苷进行细菌产量的测量更有可能反映出大量细菌种类的产生。脱氧腺苷和脱氧胞苷的掺入率分别是胸苷的0.9和0.2倍。这些结果清楚地表明，与其他脱氧核糖核苷相比，可以将外源性脱氧鸟苷掺入其DNA的细菌种类的数量相对更大，并且使用脱氧鸟苷进行细菌产量的测量更有可能反映出大量细菌种类的产生。脱氧腺苷和脱氧胞苷的掺入率分别是胸苷的0.9和0.2倍。这些结果清楚地表明，与其他脱氧核糖核苷相比，可以将外源性脱氧鸟苷掺入其DNA的细菌种类的数量相对更大，并且使用脱氧鸟苷进行细菌产量的测量更有可能反映出大量细菌种类的产生。

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Incorporation characteristics of exogenous 15N-labeled thymidine, deoxyadenosine, deoxyguanosine and deoxycytidine into bacterial DNA.

Bacterial production has been often estimated from DNA synthesis rates by using tritium-labeled thymidine. Some bacteria species cannot incorporate extracellular thymidine into their DNA, suggesting their biomass production might be overlooked when using the conventional method. In the present study, to evaluate appropriateness of deoxyribonucleosides for evaluating bacterial production of natural bacterial communities from the viewpoint of DNA synthesis, incorporation rates of four deoxyribonucleosides (thymidine, deoxyadenosine, deoxyguanosine and deoxycytidine) labeled by nitrogen stable isotope (15N) into bacterial DNA were examined in both ocean (Sagami Bay) and freshwater (Lake Kasumigaura) ecosystems in July 2015 and January 2016. In most stations in Sagami Bay and Lake Kasumigaura, we found that incorporation rates of deoxyguanosine were the highest among those of the four deoxyribonucleosides, and the incorporation rate of deoxyguanosine was approximately 2.5 times higher than that of thymidine. Whereas, incorporation rates of deoxyadenosine and deoxycytidine were 0.9 and 0.2 times higher than that of thymidine. These results clearly suggest that the numbers of bacterial species which can incorporate exogenous deoxyguanosine into their DNA are relatively greater as compared to the other deoxyribonucleosides, and measurement of bacterial production using deoxyguanosine more likely reflects larger numbers of bacterial species productions.

更新日期：2020-02-28

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