In this work, a novel bright yellow fluorescent carbon dots (CDs) was synthesized from N-methyl-1,2-phenylenediamine hydrochloride in ethanol solvent through the solvothermal method. The obtained carbon dots had no fluorescence in water, but could specifically light up in the cell which makes the staining process without washing. Interestingly, the imaging process can be performed by simply shaking the culture with the cells for only 1 min, indicating ultrafast and easy to operate fluorescence imaging. Moreover, the carbon dots with abundant amino functional groups had good lysosome targeting properties (the Pearson's correlation coefficient is 0.92) and could achieve the ultrafast lysosome imaging in cell and zebrafish and monitoring cell apoptosis status. This is the first lysosome targeting carbon dots that allows the combination of a short incubation at the second-level with wash-free process providing great potential for continuous observation lysosome in vivo.

中文翻译：

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一种用于超快速成像和监测细胞凋亡状态的靶向碳点的免洗溶酶体

在这项工作中，通过溶剂热法从 N-甲基-1,2-苯二胺盐酸盐在乙醇溶剂中合成了一种新型的亮黄色荧光碳点 (CDs)。得到的碳点在水中没有荧光，但可以在细胞内特异性发光，使染色过程无需洗涤。有趣的是，只需将培养物与细胞一起摇晃 1 分钟即可完成成像过程，这表明荧光成像超快且易于操作。此外，具有丰富氨基官能团的碳点具有良好的溶酶体靶向特性（Pearson 相关系数为 0.92），可以实现细胞和斑马鱼的超快溶酶体成像和监测细胞凋亡状态。